Abstract
Considerable attention has been directed in recent years toward tumor cell surface sialomucins (Carraway and Spielman, 1986). The presence of these O-glycosylated glycoproteins has been correlated with metastasis in the 13762NF rat mammary adenocarcinoma (Steck and Nicolson, 1983), and they are proposed to protect carcinomas from immune destruction by “masking” cell surface antigens, including histocompatibility antigens (Codington, 1981). Monoclonal antibodies prepared against whole tumor cells (Lan et al., 1985), tumor cell membranes (Kufe et al., 1984) or human milk fat globule membranes (Taylor-Papadimitriou, 1981) in many cases react specifically with carcinoma cell sialomucins and are being investigated for their potential in diagnosis and therapy (Schlom, 1986). Why should these mucin-like molecules, which are also products of normal tissues (Ceriani et al., 1983), be recognized as “carcinoma-associated antigens”? One possibility is that changes in the glycosylation of these glycoproteins in carcinomas yields epitopes which are absent or uncommon in normal tissues or other tumors (Hull and Carraway, 1988).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Carraway, K. L. and Hull, S. R., 1989, O-Glycosylation pathway for mucin-type glycoproteins, BioEssays, in press.
Carraway, K. L., and Spielman, J., 1986, Structural and functional aspects of tumor cell sialomucins, Molec. Cell. Biochem., 72:109–120.
Ceriani, R. L., Peterson, J. A., Lee, J. Y., Moncada, R., and Blank, E. W., 1983, Characterization of cell surface antigens of human mammary epithelial cells with monoclonal antibodies prepared against human milk fat globule, Somatic Cell Genetics, 9:415–427.
Codington, J. F., 1981, Masking of cancer cell surface antigens, Handbook of Cancer Immunol., 8:171–203.
Helm, R. M., and Carraway, K.L., 1981, Evidence for the association of two cell surface glycoproteins of 13762 mammary ascites tumor cells, Exp. Cell Res., 135:418–424.
Hull, S. R., and Carraway, K. L., 1988, Mechanism of expression of Thomsen-Friedenreich (T) antigen at the cell surface of a mammary adenocarcinoma, FASEB J., 2:2380–2384.
Hull, S. R., and Carraway, K. L., 1989, Sulfation of the tumor cell surface sialomucin of the 13762 rat mammary adenocarcinoma, J. Cell. Biochem., in press. Kufe, D., Inghirami, G., Abe, M., Hayes, D., Justi-Wheeler, H., and Schlom, J., 1984, Differential reactivity of a novel monoclonal antibody (DF3) with human malignant versus benign breast tumors, Hybridoma, 3:223–232.
Lan, M. S., Finn, O. J., Fernstein, P. D., and Metzgar, R.S., 1985, Isolation and properties of a human pancreatic adenocarcinoma-associated antigen, Cancer Res., 45: 305–310.
Lodish, H. F., 1988, Transport of secretory and membrane glycoproteins from the rough endoplasmic reticulum to the Golgi. A rate-limiting step in protein maturation and secretion, J. Biol. Chem., 263:2107–2110.
Schlom, J., 1986, Basic principles and applications of monoclonal antibodies in the management of carcinomas, Cancer Res., 46:3235–3238.
Sherblom, A. P., Buck, R. L., and Carraway, K.L., 1980, Purification of the major sialoglycoproteins of 13762 MAT-B1 and MAT-C1 rat mammary adenocarcinoma cells by density gradient centrifugation in cesium chloride and guanidine hydrochloride, J. Biol. Chem., 255:783–790.
Spielman, J., Rockley, N. L., and Carraway, K. L., 1987, Temporal aspects of O-glycosylation and cell surface expression of ascites sialoglycoprotein-1, the major cell surface sialomucin of 13762 mammary ascites tumor cells, J. Biol. Chem., 262:269–275.
Spielman, J., Hull, S. R., Sheng, Z., Kanterman, R., Bright, A., and Carraway, K. L., 1988, Biosynthesis of a tumor cell surface sialomucin. Maturation and effects of monensin, J. Biol. Chem., 263:9621–9629.
Springer, G. F., 1984, T and Tn, general carcinoma autoantigens, Science, 224:1198–1206.
Steck, P. A., and Nicolson, G. L., 1983, Cell surface glycoproteins of 13762NF mammary adenocarcinoma clones of differing metastatic potentials, Exp. Cell Res., 147:255–267.
Tartakoff, A. M., 1983, Perturbation of vesicular traffic with the carboxylic ionophore monensin, Cell, 32:1026–1028.
Taylor-Papadimitriou, J., Peterson, J. A., Arklie, J., Burchell, J., Ceriani, R. L., and Bodmer, W.F., 1981, Monoclonal antibodies to epithelial-specific components of the human milk globule membrane: Production and reaction with cells in culture, Int. J. Cancer, 28:17–21.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Hull, S.R., Spielman, J., Carraway, K.L. (1989). Biosynthesis of the Cell Surface Sialomucin from Ascites 13762 Rat Mammary Adenocarcinoma Cells: Intracellular Maturation. In: Ceriani, R.L. (eds) Breast Cancer Immunodiagnosis and Immunotherapy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1296-4_7
Download citation
DOI: https://doi.org/10.1007/978-1-4757-1296-4_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-1298-8
Online ISBN: 978-1-4757-1296-4
eBook Packages: Springer Book Archive