Differential Expression of DF3 Antigen between Papillary Carcinomas and Benign Papillary Lesions of the Breast
Murine monoclonal antibody (MAb) DF3, prepared against a membraneenriched fraction of breast carcinoma metastasis, has previously shown differential reactivity to breast carcinoma cells and normal or benign breast epithelial cells. Using the avidin-biotin peroxidase complex immunohistochemical method, we have examined cytoplasmic DF3 antigen expression in breast papillary lesions to define whether the MAb DF3 distinguishes papillary carcinomas from benign papillary lesions. MAb DF3 reacted with antigen in the cytoplasm in 17 of 19 papillary carcinomas, 12 (63%) of which demonstrated significant levels of cytoplasmic DF3 expression (≥20% of carcinoma cells reactive). In contrast, none of the benign lesions examined demonstrated more than 10% of epithelial cells reactive in the cytoplasm. Specimens containing a spectrum of lesions from benign papilloma through atypical hyperplasia to carcinoma showed a corresponding general increase in DF3 antigen expression in the cytoplasm with the transition of papilloma to carcinoma. These results suggest that papillary carcinomas express DF3 antigen in the cytoplasm, and this may help to distinguish them from benign papillary lesions on the basis of cytoplasmic reactivity.
KeywordsPapillary Carcinoma Atypical Hyperplasia Epithelial Hyperplasia Papillary Lesion Intraductal Papilloma
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