Abstract
A cell is described that has enabled isolated Langendorff-perfused ferret hearts to be studied in a Bruker WM200 widebore superconducting nuclear magnetic resonance (NMR) spectrometer. Left ventricular pressure was monitored with a latex balloon catheter, and the hearts were paced with a stimulator triggered from the spectrometer’s central computer, enabling gated studies to be performed. Suitable radiofrequency filtering for the pacing leads is described. Phosphorus (31P) NMR was used to determine internal pH and the concentration of phosphorylated metabolites under resting conditions. The perfusion rate is shown to affect the phosphocreatine/ATP ratio at low flow rates, but the removal of phosphate from the perfusate is shown not to affect metabolite levels or the internal pH. The time resolution of the method is assessed and its potential for monitoring transient effects illustrated by studies of the effects of acetylcholine and cyanide-induced anoxia. The cardiac gated 31P NMR experiment is discussed and four spectra, corresponding to mid- and end systole and mid- and end diastole are presented. No effects of cycling of high-energy phosphates are evident in these results.
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References
Hoult, D. I., Busby, S. J. W., Gadian, D. G., Radda, G. K., Richards, R. E., and Seeley, P. J. 1974. Observation of tissue metabolites using P-31 nuclear magnetic resonance. Nature (London) 252:285–287.
Jacobus, W. E., Taylor, G. J., IV, Hollis, D. P., and Nunnally, R. L. 1977. Phosphorus nuclear magnetic resonance of perfused working rat hearts. Nature (London) 265:756–758.
Garlick, P. B., Radda, G. K., Seeley, P. J., and Chance, B. 1977. Phosphorus NMR studies on perfused heart. Biochem. Biophys. Res. Commun. 74:1256–1262.
Ingwall, J. S. 1982. Phosphorus nuclear magnetic resonance spectroscopy of cardiac and skeletal muscles. Am. J. Physiol. 242:H729–H744.
Dawson, M. J. 1983. Nuclear magnetic resonance. In: A. J. Drake-Holland and M. I. M. Noble (eds.), Cardiac Metabolism. pp. 309–337. Wiley and Sons, Chichester.
Dawson, M. J., and Wilkie, D. R. 1983. Muscle and brain metabolism studied by 31P nuclear magnetic resonance. In: P. Baker (ed.), Recent Advances in Physiology. pp. 247–276. Churchill Livingstone, Edinburgh.
Neurohr, K. J., Barrett, E. J., and Shulman, R. G. 1983. In vivo carbon-13 nuclear magnetic resonance studies of heart metabolism. Proc. Natl. Acad. Sci. U.S.A. 80:1603–1607.
Moon, R. B., and Richards, J. H. 1973. Determination of intracellular pH as observed by 31-P magnetic resonance. J. Biol. Chem. 248:7276–7278.
Allen, D. G., and Orchard, C. H. 1983. Intracellular calcium concentration during hypoxia and metabolic inhibition in mammalian ventricular muscle. J. Physiol. 339:107–122.
Salhany, J. M., Pieper, G. M. Wu, S., Todd, G. L., Clayton, F. C., and Eli, R. S. 1979. P-31 nuclear magnetic resonance measurement of cardiac pH in perfused guinea-pig hearts. J. Mol. Cell Cardiol. 11:601–610.
Matthews, P. M. 1982. Applications of NMR to the study of cardiac metabolism. Ph.D. thesis, Oxford.
Gupta, R. K., Benovic, J. L., and Rose, Z. B. 1978. The determination of the free magnesium level in the human red blood cell by P-31 NMR. J. Biol. Chem. 253:6172–6176.
Morris, P. G., Burgen, A. S. V., Harbird, C. J., and Callingham, B. W. 1982. Unpublished results.
Fossel, E. T., Morgan, H. E., and Ingwall, J. S. 1980. Measurement of changes in highenergy phosphates in the cardiac cycle using gated P-31 NMR. Proc. Natl. Acad. Sci. U. S.A . 77:3654–3658.
Mansfield, P., and Morris, P. G. 1982. NMR Imaging in Biomedicine. Advances in Magnetic Resonance, Supplement 2. Academic Press, New York.
Matthews, P. M., Williams, S. R., Seymour, A. M., Schwartz, A., Dube, G., Gadian, D. G., and Radda, G. K. 1982. A phosphorus-31 NMR study of some metabolic and functional effects of the inotropic agents epinephrine and ouabain, and the ionophore Ro2–2985 (X537A) in the isolated perfused rat heart. Biochim. Biophys. Acta 720:163–171.
La Noue, K. F., and Schoolwerth, A. C. 1979. Metabolic transport in mitochondria. Annu. Rev. Biochem. 48:871–922.
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Morris, P.G., Allen, D.G., Orchard, C.H. (1985). High-Time-Resolution 31P NMR Studies of the Perfused Ferret Heart. In: Harris, P., Poole-Wilson, P.A. (eds) Advances in Myocardiology. Advances in Myocardiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1287-2_3
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DOI: https://doi.org/10.1007/978-1-4757-1287-2_3
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