The Inducible Immunity System of Giant Silk Moths
In this paper we intend to summarize briefly the main findings from our work on the inducible defense system of giant silk moths. This immunity manifests itself as a potent, cell-free antibacterial activity in the hemolymph (Boman et al., 1974a). A key tool for us has been an in vitro assay of bacterial killing measured by the loss of the colony forming ability. Our main test organism has been Escherichia coli, D31 (Boman et al., 1974). This strain carries resistance to streptomycin and ampicillin, properties which we have used both to eliminate any contaminating bacteria and for selective plating during double infections. Some of the parameters of our assay as well as its antibacterial spectrum have recently been discussed elsewhere (Rasmuson and Boman, 1977).
KeywordsKilling Activity Ammonium Sulfate Fraction Physiological Salt Solution Immune Protein Killing Assay
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