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Sedimentation Equilibrium of Proteins in Density Gradients

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Methods of Protein Separation

Part of the book series: Biological Separations ((BIOSEP))

Abstract

The technique of equilibrium density-gradient ultracentrifugation in the analytical ultracentrifuge was first described by Meselson, Stahl, and Vinograd in 1957 (Meselson et al., 1957). The method provides a thermodynamically rigorous procedure for the determination of the buoyant density, hydration, and solvated molecular weight of polymers having molecular weights larger than about 10,000.

This work was supported in part by a research grant from the National Institute of General Medical Sciences (GM 18871), National Institutes of Health, U.S. Public Health Service.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Redlands, Redlands, California, 92373, USA

    James B. Ifft

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  1. James B. Ifft
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Editor information

Editors and Affiliations

  1. Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Nicholas Catsimpoolas

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Ifft, J.B. (1975). Sedimentation Equilibrium of Proteins in Density Gradients . In: Catsimpoolas, N. (eds) Methods of Protein Separation. Biological Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1257-5_7

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  • DOI: https://doi.org/10.1007/978-1-4757-1257-5_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1259-9

  • Online ISBN: 978-1-4757-1257-5

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