Chromatographic Peak Shape Analysis

  • Eli Grushka
Part of the Biological Separations book series (BIOSEP)


The shape of chromatographic peak is of utmost importance since it can inform the scientist of the processes which occur in the column where the separation is taking place. Consequently, the shape can yield a wealth of information dealing with such diverse topics as the recognition of strongly overlapped peaks and adsorption—desorption rate constants. It is, therefore, surprising that the majority of the papers dealing with chromatographic theory discuss mainly retention characteristics and/or zone broadening. The peak shape is fundamentally more important since not only can the retention time and the peak width be obtained from it, but the validity of the usual assumption made in chromatography (namely, Gaussian shape; infinitely fast mass transfer through the stationary—mobile phase interface) can be ascertained by analyzing the shape.


Peak Shape American Chemical Society Height Ratio Double Peak Central Moment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ashley, J. W. and Reilly, C. N. (1965) Anal. Chem. 37: 626.CrossRefGoogle Scholar
  2. Bocke, J. and Parke, N. G., III (1962) in Gas Chromatography (N. Brenner, ed.) p. 391, Academic Press, New York.Google Scholar
  3. Buys, T. S. and de Clerk, K. (1972a) J. Chromatogr. 67: 1.CrossRefGoogle Scholar
  4. Buys, T. S. and de Clerk, K. (1972b) J. Chromatogr. 67: 13.CrossRefGoogle Scholar
  5. Buys, T. S. and de Clerk, K. (1972e) Sep. Sci. 7: 441.CrossRefGoogle Scholar
  6. Buys, T. S. and de Clerk, K. (1972d) Sep. Sci. 7: 543.CrossRefGoogle Scholar
  7. Buys, T. S. and de Clerk, K. (1972e) Anal. Chem. 44: 1273.CrossRefGoogle Scholar
  8. Buys, T. S. and de Clerk, K. (1972f) J. Chromatogr. Sci. 10: 722.Google Scholar
  9. Catsimpoolas, N. and Griffith, A. L. (1973) Anal. Biochem. 56: 100.PubMedCrossRefGoogle Scholar
  10. Catsimpoolas, N., Yotis, W. W., Griffith, A. L., and Rodbard, D. (1974) Arch. Biochem. Biophys. 163: 113.PubMedCrossRefGoogle Scholar
  11. Chesler, S. N. and Cram, S. P. (1971) Anal. Chem. 43: 1922.CrossRefGoogle Scholar
  12. Chesler, S. N. and Cram, S. P. (1972) Anal. Chem. 44: 2240.CrossRefGoogle Scholar
  13. dal Nogare, S. and Chiu, J. (1962) Anal. Chem. 34: 890.CrossRefGoogle Scholar
  14. de Clerk, K. and Buys, T. S. (1971) J. Chromatogr. 63: 193.Google Scholar
  15. Funk, J. E. and Rony, P. R. (1971) Sep. Sci. 6: 365.CrossRefGoogle Scholar
  16. Giddings, J. C. (1965) Dynamics of Chromatography,Marcel Dekker, New York, New York.Google Scholar
  17. Giese, A. T. and French, C. S. (1955) Appl. Spectrosc. 9: 78.CrossRefGoogle Scholar
  18. Glueckauf, E. (1955) Trans. Faraday Soc. 51: 34.CrossRefGoogle Scholar
  19. Grubner, O. (1968) in Advances in Chromatography (R. A. Keller and J. C. Giddings, eds.) p. 173, Marcel Dekker, New York.Google Scholar
  20. Grubner, O. (1971) Anal. Chem. 43: 1934.CrossRefGoogle Scholar
  21. Grubner, O. and Underhill, D. W. (1970) Sep. Sci. 5: 555.CrossRefGoogle Scholar
  22. Grubner, O. and Underhill, D. W. (1972) J. Chromatogr. 73: 1.CrossRefGoogle Scholar
  23. Grubner, O., Ralek, M., and Zikenova, A. (1966a) Collect. Czech. Cohem. Commun. 31: 852.Google Scholar
  24. Grubner, O., Ralek, M., and Kucera, E. (1966b) Collect. Czech. Chem. Commun. 31: 2629.Google Scholar
  25. Grubner, O., Zikenova, A., and Ralek, M. (1967) J. Chromatogr. 28: 209.PubMedCrossRefGoogle Scholar
  26. Grushka, E. (1972a) J. Phys. Chem. 76: 2586.CrossRefGoogle Scholar
  27. Grushka, E. (1972b) Anal. Chem. 44: 1733.PubMedCrossRefGoogle Scholar
  28. Grushka, E. and Monacelli, G. C. (1972) Anal. Chem. 44: 484.CrossRefGoogle Scholar
  29. Grushka, E., Myers, M. N., Schettler, P. D., and Giddings, J. C. (1969) Anal. Chem. 41: 889.CrossRefGoogle Scholar
  30. Grushka, E., Marcus, M. N., and Giddings, J. C. (1970) Anal. Chem. 42: 21.CrossRefGoogle Scholar
  31. James, A. T. and Martin, A. J. P. (1952) Biochem. J. 50: 679.PubMedGoogle Scholar
  32. James, M. R., Giddings, J. C., and Eyring, H. (1964) J. Phys. Chem. 68: 725.Google Scholar
  33. Kambara, T. and Saitah, K. (1968) J. Chromatogr. 35: 318.Google Scholar
  34. Kaminiskii, V. A., Timoshev, S. F., and Tunitskii, N. N. (1965) Russ. J. Phys. Chem 39: 1354.Google Scholar
  35. Kocisik, M. (1967) J. Chromatogr. 30: 459.CrossRefGoogle Scholar
  36. Kubin, M. (1965) Collec. Czech. Chem. Comun. 30: 1104.Google Scholar
  37. Kucera, E. (1965) J. Chromatogr. 19: 237.PubMedCrossRefGoogle Scholar
  38. Lapidus, L. and Amundson, N. R. (1952) J. Phys. Chem. 56: 984.CrossRefGoogle Scholar
  39. Leathard, D. A. and Sherlock, B. C. (1970) in Identification Techniques in Gas Chromatography, Wiley-Interscience, New York.Google Scholar
  40. McNair, H. M. and Cooke, W. M. (1972) J. Chromatogr. Sci. 10: 27.Google Scholar
  41. McQuarrie, D. A. (1963) J. Chem. Phys. 38; 437.CrossRefGoogle Scholar
  42. McWilliam, I. G. (1969) Anal. Chem. 41: 674.CrossRefGoogle Scholar
  43. McWilliam, I. G. and Bolton, H. C. (1969) Anal. Chem. 41: 1755.CrossRefGoogle Scholar
  44. McWilliam, I. G. and Bolton, H. C. (1971) Anal. Chem. 43: 883.CrossRefGoogle Scholar
  45. Martin, A. J. P. and Synge, R. L. M. (1941) Biochem. J. 35: 1358.PubMedGoogle Scholar
  46. Mehta, R. V., Merson, R. L., and McCoy, B. J. (1974) J. Chromatogr. 88: 1.CrossRefGoogle Scholar
  47. Nielson, K. L. (1964) in Methods in Numerical Analysis, 2nd ed., Macmillan, New York.Google Scholar
  48. Oberholtzer, J. E. and Rogers, L. B. (1969) Anal. Chem. 44: 1234.CrossRefGoogle Scholar
  49. Riedmann, M. (1969) Ber. Bunsenges. Phys. Chem. 69: 840.Google Scholar
  50. Rony, P. R. and Funk, J. E. (1971) Sep. Sci. 6: 383.CrossRefGoogle Scholar
  51. Steinberg, J. C. (1966) in Advances in Chromatography (J. C. Giddings and R. A. Keller, eds.) p. 205, Marcel Dekker, New York.Google Scholar
  52. Suzuki, M. and Smith, J. M. (1975) in Advances in Chromatography, Vol. 13, Marcel Dekker, New York.Google Scholar
  53. van Deemter, J. J., Zuiderweg, F. J., and Klinkenberg, A. (1956) Chem. Eng. Sci. 5: 271.CrossRefGoogle Scholar
  54. Villermaux, J. (1973) J. Chromatogr. 83: 205.CrossRefGoogle Scholar
  55. Vink, H. (1965) J. Chromatogr. 20: 305.CrossRefGoogle Scholar
  56. Yamaska, K. and Nakagawa, T. (1973) J. Chromatogr. 93: 1.Google Scholar
  57. Yamaska, K. and Nakagawa, T. (1974) J. Chromatogr. 92: 213.CrossRefGoogle Scholar
  58. Yamazaki, H. (1967) J. Chromatogr. 27: 14.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Eli Grushka
    • 1
  1. 1.Department of ChemistryState University of New York at BuffaloBuffaloUSA

Personalised recommendations