Purification of Chemically Modified Proteins

  • Robert E. Feeney
  • David T. Osuga
Part of the Biological Separations book series (BIOSEP)


Protein chemists have long been interested in changing the chemical, physical, and biological properties of proteins by chemically altering their structure. In recent years, the application of modern knowledge of proteins and the uses of new chemical reagents and more sophisticated analytical techniques have made chemical modifications of proteins a major tool in protein chemistry (Hirs and Timasheff, 1972a; Means and Feeney, 1971). But, even with the availability of modern procedures, purification and analysis of the chemically modified products still remain as formidable tasks in using chemical modification in protein chemistry.


Disulfide Bond Chemical Modification Iodoacetic Acid Performic Acid Antifreeze Glycoprotein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Ahmed, A. 1., Osuga, D. T., and Feeney, R. E. (1973) J. Biol. Chem. 248: 8524.PubMedGoogle Scholar
  2. Ako, H., Foster, R. J., and Ryan, C. A. (1974) Biochemistry 13: 132.PubMedCrossRefGoogle Scholar
  3. Blankenhorn, G., Osuga, D. T., Lee, H. S., and Feeney, R. E. (1975) Biochim. Biophys. Acta: in press. Bohak, Z. (1964) J. Biol. Chem. 239: 2878.Google Scholar
  4. Brewer, C. F. and Riehm, J. P. (1967) Anal. Biochem. 18: 248.CrossRefGoogle Scholar
  5. Cavallini, D., Federici, G., Barboni, E., and Marcucci, M. (1970) FEBS Lett. 10: 125.PubMedCrossRefGoogle Scholar
  6. Cuatrecasas, P. and Anfinsen, C. B. (1971) Annu. Rev. Biochem. 40: 259.PubMedCrossRefGoogle Scholar
  7. Davies, G. E. and Stark, G. R. (1970) Proc. Natl. Acad. Sci. U.S.A 66: 651.PubMedCrossRefGoogle Scholar
  8. DeVries, A. L., Komatsu, S. K., and Feeney, R. E. (1970) J. Biol. Chem. 245: 2901.Google Scholar
  9. Dixon, H. B. F. and Thompson, C. M. (1968) Biochem. J. 107: 427.PubMedGoogle Scholar
  10. Downs, F. and Pigman, W. (1969) Biochemistry 8: 1760.PubMedCrossRefGoogle Scholar
  11. Er-el, Z., Zaidenzaig, Y., and Shaltiel, S. (1972) Biochem. Biophys. Res. Commun. 49: 383.PubMedCrossRefGoogle Scholar
  12. Fasold, H., Klappenberger, J., Meyer, C., and Remold, H. (1971) Angew. Chem. 10: 795.CrossRefGoogle Scholar
  13. Feeney, R. E. (1974) Am. Sci. 62: 712.PubMedGoogle Scholar
  14. Feeney, R. E., Blankenhorn, G., and Dixon, H. B. F. (1975) Adv. Protein Chem. 29: 135.PubMedCrossRefGoogle Scholar
  15. Feinstein, G. (1970) FEBS Lett. 7: 353.PubMedCrossRefGoogle Scholar
  16. Feinstein, G. (1971) Naturwissenschaften 58: 389.PubMedCrossRefGoogle Scholar
  17. Feinstein, G. and Feeney, R. E. (1967) Biochim. Biophys. Acta 140: 55.CrossRefGoogle Scholar
  18. Gibbons, I., Yang, Y. R. and Schachman, H. K. (1974) Proc. Natl. Acad. Sci. U.S.A. 71: 4452.PubMedCrossRefGoogle Scholar
  19. Haynes, R., Osuga, D. T. and Feeney, R. E. (1967) Biochemistry 6: 541.PubMedCrossRefGoogle Scholar
  20. Hirs, C. H. and Timasheff, S. N. (1972a) Meth. Enzymol. 25B: 1.Google Scholar
  21. Hirs, C. H. and Timasheff, S. N. (1972b) Meth. Enzymol. 26C: 1.Google Scholar
  22. Hirs, C. H. and Timasheff, S. N. (1973) Meth. Enzymol. 27D: 1.Google Scholar
  23. Illingworth, J. A. (1972) Biochem. J. 129: 1119.PubMedGoogle Scholar
  24. Laskowski, M. and Sealock, R. W. (1971) in Enzymes 3rd ed. 3 (P. D. Boyer, ed) pp. 375473, Academic Press, New York.Google Scholar
  25. Lis, H. and Sharon, N. (1973) Annu. Rev. Biochem. 42: 541.PubMedCrossRefGoogle Scholar
  26. Liu, W. H., Feinstein, G., Osuga, D. T., Haynes, R., and Feeney, R. E. (1968) Biochemistry 7: 2886.Google Scholar
  27. Means, G. E. and Feeney, R. E. (1968) Biochemistry 7: 2192.Google Scholar
  28. Means, G. E. and Feeney, R. E. (1971) Chemical Modification of Proteins, Holden-Day, San Francisco.Google Scholar
  29. Means, G. E., Ryan, D. S., and Feeney, R. E. (1974) Acc. Chem. Res. 7: 315.CrossRefGoogle Scholar
  30. Mohammadzadeh-K., A., Smith, L. M., and Feeney, R. E. (1969) Biochim. Biophys. Acta 194: 256.CrossRefGoogle Scholar
  31. Nakagawa, Y. and Bender, M. L. (1970) Biochemistry 9: 259.PubMedCrossRefGoogle Scholar
  32. Osuga, D. T., Bigler, J. C., Uy, R. L., Sjoberg, L., and Feeney, R. E. (1974) Comp. Biochem. Physiol. 48B: 519.Google Scholar
  33. Rae, P. A. and Schimmer, B. P. (1974) J. Biol. Chem. 249: 5649.PubMedGoogle Scholar
  34. Rando, R. (1974) Science 185: 320.PubMedCrossRefGoogle Scholar
  35. Ray, W. J., Jr. and Koshland, D. E., Jr. (1962) J. Biol. Chem. 237: 2493.PubMedGoogle Scholar
  36. Rice, R. H. and Means, G. E. (1971) J. Biol.Chem. 246: 831.PubMedGoogle Scholar
  37. Ryan, D. S. and Feeney, R. E. (1974) Bayer Symp. V: 378.CrossRefGoogle Scholar
  38. Ryan, D. S. and Feeney, R. E. (1975) J. Biol. Chem. 250: 843.Google Scholar
  39. Schwimmer, S., Bevenue, A., and Weston, W. J. (1956) Arch. Biochem. Biophys. 60: 279.PubMedCrossRefGoogle Scholar
  40. Shaltiel, S. (1973) in Metabolic Interconversion of Enzymes (E. H. Fischer, E. H. Krebs, H. Neurath, and E. R. Stadtman, eds.) pp. 379 - 392, Springer-Verlag, Berlin.Google Scholar
  41. Stevens, F. C. and Feeney, R. E. (1963) Biochemistry 2: 1346.CrossRefGoogle Scholar
  42. Tschesche, H., Jering, H., Schorp, G., and Dietl, T. (1974) Bayer Symp. V: 362.CrossRefGoogle Scholar
  43. Vandenheede, J. R. (1972) Primary Structure and Mechanism of Action of a Freezing - Point Depressing Glycoprotein from Antarctic Fish. Ph.D. Thesis, University of California, Davis, Calif.Google Scholar
  44. Wilchek, M. and Givol, D. (1973) in Peptides (H. Nesvaba, ed.) pp. 203–222, North-Holland, Amsterdam.Google Scholar
  45. Wilchek, M., Bocchini, V., Becker, M., and Givol, D. (1971) Biochemistry 10: 2828.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Robert E. Feeney
    • 1
  • David T. Osuga
    • 1
  1. 1.Department of Food Science and TechnologyUniversity of CaliforniaDavisUSA

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