• Alfred J. Crowle
Part of the Biological Separations book series (BIOSEP)


Immunodiffusion is an analytic technique in which reactants diffuse to intermingle with each other and react immunologically. Generally, the primary reagent will be antiserum and the substance to be analyzed will be antigen; but sometimes these roles are reversed, antigen being used to study antibodies. Immunodiffusion tests are performed in semisolid media, usually gels, to allow reactants to diffuse while preventing convective mixing, and to support accumulation of the antigen–antibody reaction product, usually a precipitate, for observation.


Partial Identity Antigen Molecule Native Antigen Immunodiffusion Test Serum Antigen 
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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Alfred J. Crowle
    • 1
  1. 1.Webb-Waring Lung Institute and Department of MicrobiologyUniversity of Colorado School of MedicineDenverUSA

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