Advertisement

Techniques in Plant Virology

  • W. A. Stevens
Part of the Tertiary Level Biology book series (TLB)

Abstract

Let us imagine that we have been presented with diseased plant material and asked to identify the causative agent. If we also imagine that tests have shown that no fungal, bacterial or mycoplasmal pathogens are responsible for the symptoms, neither are they the result of nutrient imbalance or pest or spray damage, how then can we
  1. (a)

    determine that a virus or viruses are involved, and

     
  2. (b)

    identify the virus?

     

Keywords

Virus Particle Buoyant Density Virus Preparation Barley Stripe Mosaic Virus Partial Specific Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. Ball, E. M. (1974) Serological tests for the identification of plant viruses. Amer. Phytopath. Soc.Google Scholar
  2. Brakke, M. K. (1967) Density gradient centrifugation. In Maramorosch and Koprowski (1967)—see general section.Google Scholar
  3. Francki, R. I. B. (1980) Limited value of the thermal inactivation point, longevity in vitro, dilution end-point as criteria for the characterization, identification and classification of plant viruses. Interviroloav 13, 91–8.CrossRefGoogle Scholar
  4. Hamilton, R. I., Edwardson, J. R., Francki, R. I. B., Hsu, H. T., Hull, R., Koenig, R. and Milne, R. G. (1981) Guidelines for the identification and characterisation of plant viruses. J. Gen. Virol. 54, 223–41.CrossRefGoogle Scholar
  5. Kado, C. I. and Agrawal, H. O. (1972) Principles and Techniques in Plant Virology. Van Nostrand, Reinhold and Co.Google Scholar
  6. Koenig, R. (1981) Indirect ELISA methods for the broad specificity detection of plant viruses. J. Gen. Virol. 55, 53–62.CrossRefGoogle Scholar
  7. Markham, R. (1967) The ultracentrifuge. In Maramorosch and Koprowski (1967)—see general section.Google Scholar
  8. Milne, R. G. and Luisoni, E. (1975) Rapid high-resolution immune electron microscopy of plant viruses. Virology 68, 270–4.CrossRefGoogle Scholar
  9. Murant, A. F. and Taylor, M. (1978) Estimates of molecular weights of nepovirus RNA Species by polyacrylamide gel electrophoresis under denaturing conditions. J. Gen. Virol. 41, 53–61CrossRefGoogle Scholar
  10. Murant, A. F., Taylor, M., Duncan, G. H. and Raschke, J. H. (1981) Improved estimates of molecular weights of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation by Glyoxal. J. Gen. Virol. 53, 321–32.CrossRefGoogle Scholar
  11. Roberts, I. M. and Harrison, B. D. (1979) Detection of potato leafroll and potato mop top viruses by immunosorbent electron microscopy. Ann. appl. Biol. 93, 289–97.CrossRefGoogle Scholar
  12. Thomas, B. (1980) The detection by serological methods of viruses infecting rose. Ann. appl. Biol. 94, 91–101.CrossRefGoogle Scholar
  13. Torrance, L. and Jones, R. A. C. (1981) Recent developments in serological methods suited for routine testing for plant viruses. Plant Path. 30, 1–24.CrossRefGoogle Scholar
  14. Warmke, H. E. and Christie, R. G. (1967) The use of dilute osmium tetroxide for preservation of three dimensional crystals of TMV. Virology 32, 534–7.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • W. A. Stevens
    • 1
  1. 1.Royal Holloway CollegeUniversity of LondonUK

Personalised recommendations