ras Oncogenes pp 261-262 | Cite as

Aristolochic Acid I Induced Tumors in Wistar Rats Contain Activating Mutations in Codon 61 of the H-ras Protooncogene

  • H. H. Schmeiser
  • J. Lyons
  • J. W. G. Janssen
  • C. R. Bartram
  • H. R. Scherf
  • W. Pfau
  • M. Wiessler

Abstract

Aristolochic acid I (AAI), a nitrophenanthrene derivative, is the main compound of the naturally occurring carcinogen aristolochic acid. Previous studies showed that AAI is directly mutagenic in the Ames test (Schmeiser et al., 1984) and leads to DNA adduct formation after metabolic activation in vitro and in vivo (Schmeiser et al., 1988). After oral administration of AAI to male Wistar rats DNA-AAI adducts in forestomach, stomach, liver, kidney, bladder and lung could be detected by use of the 32P-postlabeling method.

Keywords

Aristolochic Acid Ames Test Tumorigenicity Assay 61st Codon Carcinogenic Form 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • H. H. Schmeiser
    • 1
  • J. Lyons
    • 2
  • J. W. G. Janssen
    • 2
  • C. R. Bartram
    • 2
  • H. R. Scherf
    • 1
  • W. Pfau
    • 1
  • M. Wiessler
    • 1
  1. 1.Institute of Toxicology and ChemotherapyDKFZHeidelbergGermany
  2. 2.Section of Molecular Biology, Department of Pediatrics IIUniversity of UlmUlmGermany

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