Glutathione S-Transferase/ras Fusion Protein: A Tool for Affinity Chromatography of ras Associated Proteins
Two proteins have previously been shown to interact directly with ras protein: Yeast adenylate cyclase comprizing 200 kDa and 70 kDa peptides is activated by ras protein (1) while a mammalian cytosolic protein of 125 kDa called GAP stimulates GTPase activity of normal ras protein but not that of its oncogenic varients (2). In this paper, using a ras fusion protein as a tool for affinity chromatography, I provide an evidence for the direct interaction of ras protein with at least three other proteins, i.e., tubulin, actin and cAMP-dependent protein kinase.
KeywordsFusion Protein Bovine Brain NATO Advance Research Workshop Bovine Brain Extract Major Cytoskeletal Protein
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