Suppression of ras Oncogene Expression Using Sequence Specific Oligodeoxynucleotides
The activation of ras oncogenes has been clearly implicated in the tumorigenic process, being the oncogene most frequently identified in human cancers. (Barbacid 1985). Ras genes containing point mutations as well as amplified genes leading to over-expression of the protein product p21 have been found in human tumours (Der et al 1982, Bos et al 1986). There is, however, no reason to assume that we have yet identified all of the genetic changes involved or indeed that they are necessarily the primary events in tumorigenesis.
KeywordsAntisense Oligonucleotide Tissue Culture Medium EC816 Cell Porated Cell EC816 Cell Line
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- Barbacid, M. (1985). In “Important Advances in Oncology 1986” ed. V de Vita, S. Hellman, S. Rosenberg, pp 3–22. Philadelphia Lippincot.Google Scholar
- Holt, J.T., Redner, R.L. and Neinhuis, A.W. (1987). An oligomer complementary to c-myc inhibits proliferation of HL60 promyclocytic cells and induces differentiation. Mol. Cell Biol. 8, 963.Google Scholar
- Li, Y., Seyama, T., Godison, A. K., Winokun, T. S., Lebovitz, R.M. and Lieberman, M.W. (1988). MTras T24, a metallothionein-ras fusion gene, modulates expression in cultured rat liver cells of two genes associated with in vivo liver cancer. Proc. Natl. Acad. Sci. USA 85, 344–348.PubMedCrossRefGoogle Scholar
- Linstead, P., Jennings, B., Prescott, A., Hawley, P., Warn, R., Gibson, I. (1988). Scanning electron microscopy and the transformed phenotype. Micron and Microsc. Acta 19, 155–162.Google Scholar
- McKay, I.A., Marshall, C. J., Cales, C. and Hall, A. (1986). Transformation and stimulation of DNA synthesis in NIH 3T3 cells are a titratable function of normal p21 N-ras expression. EMBO Journal 157.Google Scholar
- Reynolds V. L., Lebovitz, R. M., Warren, S., Hawley, T. S., Godwin, A.K., Lieberman, M.W. (1987). Regulation of a metallothionein-ras T24 fusion gene by zinc results in graded alterations in cell morphology and growthn.Google Scholar
- Smith, C.C., Aurelian, L., Reddy, M.P., Miller, P.S. and Ts ’O, P.O.P. (1986). Antiviral effect of an oligo (nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre mRNAs 4 and 5. Proc. Natl. Acad. Sci. USA 83 2787–2791.PubMedCrossRefGoogle Scholar
- Wickstrom, E. L., Wickstrom, E., Lyman, G.H. and Freeman, D.L. (1986). HL60 cell proliferation inhibited by an anti-c-myc pentadecadeoxynucleotide. Federation proceedings, 1708.Google Scholar
- Zamecnik, P.C., Goodchild, J., Taguchi, Y. and Sarin, P.S. (1986). Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA. Proc. Natl. Acad. Sci. USA, 83, 4143.PubMedCrossRefGoogle Scholar