ras Oncogenes pp 129-139 | Cite as

The Role of p21ras in Proliferative Signal Transduction

  • Dennis W. Stacey
  • Chun-Li Yu
  • Fu-Sheng Wei
  • Men-Hwei Tsai


While numerous evidences indicate that oncogenes or their cellular proto-oncogene counterparts play critical roles in the control of cellular proliferation, there is little indication how these molecules function or interact with each other. Our goal is to relate the biochemical and molecular characteristics of the ras proto-oncogene to its biological function within living cells. This effort relies upon a monoclonal antibody able to neutralize the biological activity of ras following microinjection into living cells. With this antibody we have effectively deprived the injected cells of ras activity and then studied the biological consequences of this treatment. The data obtained not only confirm the critical importance of ras activity during cellular proliferation; but suggests that p21 ras might have a role in the function of other proto-oncogenes. Finally, recent evidence might even indicate the means by which the activity of p2 1 ras is controlled during normal mitogenic stimulation. The implications of this possibility are discussed.


Phosphatidic Acid Proliferative Signal Mitogenic Stimulation Thymidine Labelling Active Lipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Dennis W. Stacey
    • 1
  • Chun-Li Yu
    • 1
  • Fu-Sheng Wei
    • 1
  • Men-Hwei Tsai
    • 1
  1. 1.The Department of Molecular BiologyThe Cleveland Clinic FoundationClevelandUSA

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