Suppression of Temperature-Sensitive RAS in S.cerevisiae by a Putative Protein Kinase
In the yeast Saccharomyces cerevisiae, the RAS1 and RAS2 proteins (collectively denominated RAS) are involved in the regulation of growth, metabolism, and adaptative responses (for a review, see Tamanoi, 1988). These effects are mediated, at least in part, by the adenylate cyclase/protein kinase pathway (Toda et al., 1985; Broek et al., 1985). In fact, the growth arrest caused by the inactivation of the RAS1 and RAS2 genes can be bypassed by mutations which constitutively activate the function of the adenylate cyclase (CYR1) and of three distinct but homologous cyclic AMP-dependent protein kinases (TPK1, TPK2, and TPK3) (Matsumoto et al., 1982; Toda et al., 1985; Kataoka et al., 1985; De Vendittis et al., 1986; Toda et al., 1987a-b).
KeywordsAdenylate Cyclase Catalytic Subunit Amino Acid Sequence Similarity RAS2 Gene Putative Protein Kinase
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