Abstract
Recently, specific restriction fragment length polymorphisms (RFLPs) of a number of different genomic regions have been found to be associated with increased risk for various human diseases (Caskey, 1987). RFLPs are DNA markers defined by a different pattern of restriction fragments obtained in Southern blots with genomic DNA, using probes free of repetitive sequences. Unlike classical expressed markers, RPLPs can be found in genomic sequences irrespective of whether they encode a protein or not. The possibility to approach the problem of a genetic susceptibility to cancer has been made possible by the identification and characterization of oncogenes whose somatic activation has been associated to the development of tumors (Weinberg, 1985). In fact, in addition to somatic changes in oncogene structure and/or expression which occur only in the tumor cell, the possibility must also be considered that constitutional differences in oncogene structure and regulation could influence the individual likelihood of developing tumors. This possibility is now testable since RFLPs have been detected for several of human oncogenes (Pearson et al., 1987; Biunno et al., 1988; Radice et al., 1988).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Biunno, I., Pozzi, M.R., Radice, P., Mondini, P., Pierotti, M.A., Haley, J., Waterfield, M.D. and Della Porta, G., 1988, BglII polymorphisms of the epidermal growth factor receptor (EGF-R) gene, Nucleic Acids Res., 16: 7753.
Capon, D.J., Chen, E.Y., Levinson, A.D., Seeburg, P.H. and Goeddel, D., 1983, Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue, Nature, 302: 33.
Caskey, C.T., 1987, Disease Diagnosis by Recombinant DNA Methods, Science, 230: 1223.
Ceccherini-Nelli, L., De Re, V., Molaro, G., Zilli, L., Clemente, C. and Boiocchi, M., 1983, Ha-ras-1 restriction fragment length polymorphism and susceptibility to colon adenocarcinoma, Br. J. Cancer, 56: 1.
Chandler, L.A., Ghazi, H., Jones, P.A., Boukamp, P. and Fusenig, N.E., 1987, Allele-Specific Methylation of the Human c-Ha-ras-l Gene, Cell, 50: 711.
Dracopoli, N., Houghton, A. and Lloyd, J.O., 1985, Loss of polymorphic restriction fragments in malignant melanoma: Implications for tumor heterogeneity, Proc. Natl. Acad. Sci. USA, 82: 1470.
Gerhard, D.S., Dracopoli, N.C., Bale, S.J., Houghton, A.N., Watkins, P., Payne, C.E., Greene, M.H. and Housman, D.E., 1987, Evidence against Ha-ras-l involvement in sporadic and familial melanoma, Nature, 325: 73.
Goldfarb, M., Shimizu, K., Perucho, M. and Wigler, M., 1982, Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells, Nature, 296:404.
Heighway, J., Thatcher, N., Cerny, T. and Hasleton, P.S., 1986, Genetic predisposition to human lung cancer, Br. J. Cancer, 53: 453.
Krontiris, T.G., DiMartino, N.A., Colb, M. and Parkinson, D.R., 1985, Unique allelic restriction fragments of the human Ha-ras locus in leukocyte and tumor DNAs of cancer patients, Nature, 302: 369.
Pearson, P.L., Kidd, K.K. and Willard, H.F., 1987, Report of the Committee on Human Gene Mapping by Recombinant DNA Techniques, in: “Human Gene Mapping 9”, Cytogenet. Cell Genet., 46: 390.
Pierotti, M.A., Radice, P., Biunno, I., Borrello, M.G., Cattadori, M.R. and Della Porta, G., 1986, Detection of two Tagl polymorphisms in the VTR region of the human HRAS1 oncogene, Cytogenet. Cell Genet., 43: 174.
Pierotti, M.A., Radice, P., Lacerenza, S., Mondini, P., Radice, M.T., Sozzi, G., Miozzo, M., Pilotti, S., Gasparini, M., Fossati Bellani, F. and Della Porta, G., in press, in: “Cancer Cells 7: Molecular Diagnostics of Human Cancer”, Cold Spring Harbor Laboratory, New York.
Radice, P., Pierotti, M.A., Borrello, M.G., Illeni, M.T., Rovini, D. and Della Porta, G., 1987, HRAS1 proto-oncogene polymorphisms in human malignant melanoma: TaqI defined alleles significantly associated with the disease, Oncogene, 2: 91.
Radice, P., Donghi, R., Pierotti, M.A., Longoni, A., Fusco, A., Grieco, M., Santoro, M., Vecchio, G. and Della Porta, G., 1988, RFLP for TaqI of the human thyroid papillary carcinoma (PTC) oncogene, Nucleic Acids Res., 16: 9062.
Reddy, P., 1983, Nucleotide Sequence Analysis of the T24 Human Bladder Carcinoma Oncogene, Science, 220: 1061.
Seeburg, P.H., Colby, W.W., Capon, D.J., Goeddel, D.V. and Levinson, A.D., 1984, Biological properties of human c-Ha-ras-l genes mutated at codon 12, Nature, 312: 71.
Spandidos, D.A. and Holmes, L., 1987, Transcriptional enhancer activity in the variable tandem repeat DNA sequence downstream of the human Ha-ras1 gene, FEB, 218: 41.
Thein, S.L., Oscier, D.G., Flint, J. and Wainscoat, J.S., 1986, Ha-ras hypervariable alleles in myelodysplasia, Nature, 321: 84.
Weinberg, R.A., 1985, The Action of Oncogenes in the Cytoplasm and Nucleus, Science, 230: 770.
Wyllie, F.S., Wynford-Thomas, V., Lemoine, N.R., Williams, G.T., Williams, E.D. and Wynford-Thomas, D., 1988, Ha-ras restriction fragment length polymorphisms in colorectal cancer, Br. J. Cancer, 57: 135.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Pierotti, M.A., Radice, P., Mondini, P., De Benedetti, V., Della Porta, G. (1989). Analysis of Hras1-Associated Polymorphisms and Segregation of Taq1-Defined Alleles in Different Human Tumors. In: Spandidos, D. (eds) ras Oncogenes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1235-3_10
Download citation
DOI: https://doi.org/10.1007/978-1-4757-1235-3_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-1237-7
Online ISBN: 978-1-4757-1235-3
eBook Packages: Springer Book Archive