Thrombosis pp 187-196 | Cite as

Interaction of Platelets with the Endothelium in Normal and von Willebrand Pigs

  • V. D. Fuster
  • E. J. W. Bowie
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 102)

Abstract

In order to evaluate the possible role of platelets in the development of atherosclerosis, the aortas of 11 control pigs and 11 homozygous von Willebrand (vWD) pigs were examined for spontaneous atherosclerosis. Of the 11 normal pigs, six showed multiple atherosclerotic plaques with an intimal thickening of 63 to 130 µm. In contrast, none of the von Willebrand pigs had multiple plaques and only one showed a single lesion of more than 2 mm in diameter.

In a prospective study, 11 control pigs and seven vWD pigs were given a high (2%) cholesterol diet from the age of 3 to 9 months. All of the controls developed atherosclerotic plaques; in nine the plaques exceeded 12% of the aortic surface with an intimal thickening of up to 390 µm. In contrast, four of the vWD pigs did not develop such lesions, and the lesions in two of the other pigs covered less than 8% of the aortic surface. Most of the vWD pigs did, however, develop nonatherosclerotic flat fatty lesions.

These findings, together with some preliminary findings on aortic cross-transplantation studies between control and vWD pigs, suggest the possibility of a relationship between the circulating platelet, the endothelial cell von Willebrand factor, and the reactivity of the arterial wall in the process of atherosclerosis.

Keywords

Atherosclerotic Plaque Intimal Hyperplasia High Cholesterol Diet Fibrous Plaque Factor Viii Deficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Stemerman MB, Ross R: Experimental arteriosclerosis. I. Fibrous plaque formation in primates, an electron microscope study. J EXP MED 136: 769–789, 1972.PubMedCrossRefGoogle Scholar
  2. 2.
    Ross R, Glomset J, Kariya B, Harker L: A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. PROC NAT ACAD SCI USA 71: 1207–1210, 1974.PubMedCrossRefGoogle Scholar
  3. 3.
    Harker LA, Ross R, Slichter SJ, Scott CR: Homocystine-induced arteriosclerosis: The role of endothelial cell injury and platelet response to its genesis. J CLIN INVEST 58: 731–741, 1976.PubMedCrossRefGoogle Scholar
  4. 4.
    Bowie EJW, Owen CA, Jr., Zollman PE, Thompson JH, Jr., Fass DN: Tests of hemostasis in swine: Normal values and values in pigs affected with von Willebrand’s disease. AM J VET RES 34: 1405–1407, 1973.PubMedGoogle Scholar
  5. 5.
    Mitchell JRA, Schwartz CJ: The morphology of arterial plaques. In: ARTERIAL DISEASE, Philadelphia, FA Davis Co., 1965, pp. 15–67.Google Scholar
  6. 6.
    Bowie EJW, Owen CA, Jr.: The value of measuring platelet “adhesiveness” in the diagosis of bleeding diseases. AM J CLIN PATHOL 60: 302–308, 1973.PubMedGoogle Scholar
  7. 7.
    Borchgrevink CF: Platelet adhesion in vivo in patients with bleeding disorders. ACTA MED SCAND 170: 231–243, 1961.CrossRefGoogle Scholar
  8. 8.
    Zimmerman TS, Ratnoff OD, Powell AE: Immunologic differentiation of classic hemophilia (Factor VIII deficiency) and von Willebrand’s disease: With observations on combined deficiencies of antihemophilic factor and proaccelerin (Factor V) and on acquired circulating anticoagulant against antihemophilic factor. J CLIN INVEST 50: 244–254, 1971.PubMedCrossRefGoogle Scholar
  9. 9.
    Weiss HJ, Hoyer LW, Rickles FR, Varma A, Rogers J: Quantitative assay of plasma factor deficient in von Willebrand’s disease that is necessary for platelet aggregation: Relationship to Factor VIII procoagulant activity and antigen content. J CLIN INVEST 52: 2708–2716, 1973.PubMedCrossRefGoogle Scholar
  10. 10.
    Fass DN, Brockway WJ, Owen CA, Jr., Bowie EJW: Factor VIII (Willebrand) antigen and ristocetin-Willebrand factor in pigs with von Willebrand’s disease. THROMB RES 8: 319–327, 1976.PubMedCrossRefGoogle Scholar
  11. 11.
    Weiss HJ, Tschopp TB, Baumgartner HR: Impaired interaction (adhesion-aggregation) of platelets with the subendothelium in storage-pool disease and after aspirin ingestion: A comparison with von Willebrand’s disease. N ENGL J MED 293: 619–623, 1975.PubMedCrossRefGoogle Scholar
  12. 12.
    Baumgartner HR, Tschopp TB, Meyer D: Shear rate dependance of platelet adhesion to collagenous surfaces in Willebrand factor-depleted blood. THROMB HAEMOSTAS (Abstract) 38: 50, 1977.Google Scholar
  13. 13.
    Fry DL: Certain chemorheologic considerations regarding the blood vascular interface with particular reference to coronary artery disease. CIRCULATION (Suppl) 40: 38–57, 1969.Google Scholar
  14. 14.
    Texon M: The hemodynamic concept of atherosclerosis. BULL NY ACAD MED 36: 263–274, 1960.Google Scholar
  15. 15.
    Glagov S: Mechanical stresses on vessels and the non-uniform distribution of atherosclerosis. MED CLIN NORTH AM 57: 63–77, 1973.PubMedGoogle Scholar
  16. 16.
    Wesolowski SA, Fries CC, Sabini AM, Sawyer PN: The significance of turbulence in heroic systems and in the distribution of the atherosclerotic lesion. SURGERY 57: 155–161, 1965.PubMedGoogle Scholar
  17. 17.
    Sourer JB, Evans G, Schwartz CJ: Influence of experimental aortic coarctation on the pattern of aortic Evans blue uptake in vivo. ATHEROSCLEROSIS 16: 127–133, 1972.CrossRefGoogle Scholar
  18. 18.
    Florentin RA, Nam SC: Dietary-induced atherosclerosis in miniature swine. I. Gross and light microscopy observations: Time of development and morphological characteristics of lesions. EXP MOL PATHOL 8: 263–301, 1968.PubMedCrossRefGoogle Scholar
  19. 19.
    Frost H: Study of the pathogenesis of obliterating arterial disease by means of tarter electron microscope technique. In: PLATELETS AND THE VESSEL WALL-FIBRIN DEPOSITION; Symposium of the European Atherosclerosis Group, June 15–17, 1969, edited by Schettler G. Stuttgart, Georg Thieme Verlag, 1970, p. 124.Google Scholar
  20. 20.
    Ross R, Harker L: Hyperlipidemia and atherosclerosis: Chronic hyperlipidemia initiates and maintains lesions by endothelial cell disquamation and lipid accumulation. SCIENCE 193: 1094–1100, 1976.PubMedCrossRefGoogle Scholar
  21. 21.
    Sunaga T, Yamashita Y, Numan F, Shimamoto T: Luminal surface of normal and atherosclerotic arteries observed by scanning electron microscope. In: SCANNING ELECTRON MICROSCOPY/1970, (Proceedings of the Third Annual Scanning Electron Microscope Symp. held at ITT Res. Inst, Chicago, IL ) pp. 243–248.Google Scholar
  22. 22.
    Mustard JF: Platelets, drugs, and thrombosis, the problem. In: PLATELETS, DRUGS AND THROMBOSIS: Proceedings of a Symposium held at McMaster University, Hamilton, Ontario, October 16–18, 1972, edited by Hirsch J, Cade JF, Gallus AS, Schönbaum E. New York, S. Karger, 1975, pp. 1–14.Google Scholar
  23. 23.
    Fry GL, Maca RD, Hoak JC: Enhancement of endothelial contiguity by human platelets (Abstract). CLIN RES 24: 570, 1976.Google Scholar
  24. 24.
    Gimbrone MA, Aster RH, Cotran RS, Corkery J, Jandl JH, Folkman J: Preservation of vascular integrity in organs per-fused in vitro with a platelet-rich medium. NATURE 222: 33–36, 1969.PubMedCrossRefGoogle Scholar
  25. 25.
    Wojcik JD, Van Horn DL, Webber AJ, Johnson SA: Mechanism whereby platelets support the endothelium. TRANSFUSION 9: 324–335, 1969.PubMedCrossRefGoogle Scholar
  26. 26.
    Roskam J: Du rôle de la paroi vasculaire dans l’hémostase spontanée et la pathogénie des éstats hémorragiques. THROMB DIATH HAEMORRH 12: 338–352, 1964.PubMedGoogle Scholar
  27. 27.
    Fass DN, Didisheim P, Lewis JC, Grabowski EF: Adhesion of porcine von Willebrand (vWD) platelets (Abstract). CIRCULATION (Suppl) 54: 2–116, 1976.Google Scholar
  28. 28.
    Booyse FM, Quarfoot AJ, Bell S, Fass DN, Lewis JC, Mann KG, Bowie EJW: Cultured von Willebrand porcine aortic endothelial cells: An in vitro model for studying the molecular defect(s) of the disease. PROC NATL ACAD SCI USA 74: 5702, 1977.PubMedCrossRefGoogle Scholar
  29. 29.
    Silwer J, Cronberg S, Nilsson IM: Occurrence of arteriosclerosis in von Willebrand’s disease. ACTA MED SCAND 180: 475–484, 1966.PubMedCrossRefGoogle Scholar
  30. 30.
    Fuster V, Bowie EJW, Lewis JC, Fass DN, Owen CA Jr, Brown AL: Arteriosclerosis in von Willebrand and normal pigs: Spontaneous and high cholesterol diet induced. J CLIN INVEST, 1978 (In Press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • V. D. Fuster
    • 1
  • E. J. W. Bowie
    • 1
  1. 1.Department of Cardiovascular Diseases and Hematology ResearchMayo Clinic and Mayo FoundationRochesterUSA

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