Induction of Sister Chromatid Exchange by Chemical Mutagens

  • Paul E. Perry


Cytogenetics underwent something of a revolution in the mid 1970s with the development of new techniques for the visualization of sister chromatid exchange (SCE). One of the reasons for the excitement was the observation that many chemical mutagens elicted dose dependent increases in the frequency of SCEs, and since these events proved to be simple to score and the analysis of only twenty or so cells per dose point was usually sufficient for statistical purposes, this method showed considerable advantages over the conventional cytogenetic method of assessing chromosome damage, aberration scoring. There are, however, important differences between SCE and aberrations, and this is reflected in the uses we can make of these phenomena in mutagen and carcinogen identification, and in the assessment of risk.


Sister Chromatid Exchange Chemical Mutagen Chinese Hamster Cell Cigarette Smoke Condensate Vinyl Chloride Monomer 
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  1. Allen, J. W., and Latt, S. A., Analysis of sister chromatid exchange formation in vivo in mouse spermatogonia as a new test system for environmental mutagens, Nature, 260: 449 (1976).PubMedCrossRefGoogle Scholar
  2. Allen, J. W., Shuler, C. F., and Latt, S. A., BrdU tablet methodology for in vivo studies of DNA synthesis, Somat. Cell Genet., 4: 393 (1978).CrossRefGoogle Scholar
  3. Anderson, D., Richardson, C. R., Purchase, I. E. H., Evans, H. J., and O’Riordan, M. L., Chromosomal analysis in vinyl chloride exposed workers: comparison of the standard technique with the sister chromatid exchange technique, Mutat. Res., 83: 137 (1981).PubMedCrossRefGoogle Scholar
  4. Ardito, G., Lamberti, L., Ansaldi, E., and Ponsetto, P., Sisterchromatid exchanges in cigarette-smoking human females and their newborns, Mutat. Res., 78: 209 (1980).PubMedCrossRefGoogle Scholar
  5. Carrano, A. V., Thompson, L. H., Lindl, P. A., and Minkler, J. L., Sister chromatid exchange as an indicator of mutagenesis, Nature, 271: 551 (1978).PubMedCrossRefGoogle Scholar
  6. Carrano, A. V., Thompson, L. H., Stetka, D. G., Minkler, J. L., Mazrimas, J. A., and Fong, S., DNA crosslinking, sister chromatid exchange and specific locus mutations, Mutat. Res., 63: 175 (1979).PubMedCrossRefGoogle Scholar
  7. Crossen, P. E., and Morgan, W. F., Sister chromatid exchange in cigarette smokers, Hum. Genet., 53: 425 (1980).Google Scholar
  8. Evans, H. J., and Vijayalaxmi, Induction of 8-azaguanine resistance and sister chromatid exchange in human lymphocytes exposed to mitomycin-C and x-rays in vitro, Nature, 292: 601 (1981).Google Scholar
  9. Funes-Craviot, F., Kolmodin-Hedman, B., Lindsten, J., Nordenskjold, M., Apata Gayon, G., Lambert, B., Norberg, G., Olin, R., and Swenson, A., Chromosome aberrations and sister chromatid exchanges in workers in chemical laboratories and a rotoprinting factory and in children of women laboratory workers, Lancet, ii: 322 (1977).Google Scholar
  10. Galloway, S. M., Perry, P. E. Meneses, J., Nebert, D. W., and Pedersen, R. A., Cultured mouse embryos metabolize benzo(a)pyrene during early gestation: genetic differences detectable by sister chromatid exchanges, Proc. Natl. Acad. Sci. USA, 77: 3524 (1980).CrossRefGoogle Scholar
  11. Hollander, D. H., Tockman, M. S., Liang, Y. W., Borgaonkar, D. S., and Frost, J. K., Sister chromatid exchanges in the peripheral blood of cigarette smokers and in lung cancer patients and the effect of chemotherapy, Hum. Genet., 44: 167 (1978).Google Scholar
  12. Hopkin, J. M., and Evans, H. J., Cigarette smoke-induced DNA damage and lung cancer risks, Nature, 283: 338 (1980).CrossRefGoogle Scholar
  13. Hopkin, J. M., and Perry, P. E., Benzo(a)pyrene does not contribute to the SCEs induced by cigarette smoke condensate, Mutat. Res., 77: 377 (1980).Google Scholar
  14. Ishii, Y., and Bender, M., Factors influencing the frequency of mitomycin C-induced sister chromatid exchanges in 5-bromodeoxyuridine substituted human lymphocytes in culture, Mutat. Res., 51: 411 (1978).Google Scholar
  15. Jensen, E. M., LaPolla, R. J., Kirby, P. E., and Harworth, S. R., In vitro studies of chemical mutagens and carcinogens, I. Stability studies in cell culture medium, J. Natl. Cancer Inst., 59: 941 (1977).PubMedGoogle Scholar
  16. Krishna Murthy, P. B., and Prema, K., Sister-chromatid exchanges in oral contraceptive users, Mutat. Res., 68: 149 (1979).Google Scholar
  17. Kucerova, M., Polivkova, Z., and Batora, J., Comparative evaluation of the frequency of chromosomal aberrations and the sister chromatid exchange numbers in peripheral lymphocytes of workers occupationally exposed to vinyl chloride monomer, Mutat. Res., 67: 97 (1979).Google Scholar
  18. Lambert, B., Linblad, A., Nordenskjold, M., and Werelius, B., Increased frequency of sister chromatid exchanges in cigarette smokers, Hereditas, 88: 147 (1978).PubMedCrossRefGoogle Scholar
  19. Lambert, B., Ringborg, U., and Lindbad, A., Prolonged increase of sister-chromatid exchanges in lymphocytes of melanoma patients after CCNU treatment, Mut. Res., 59: 295 (1979).Google Scholar
  20. Latt, S. A., Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes, Proc. Natl. Acad. Sci. USA, 70: 3395 (1973).PubMedCrossRefGoogle Scholar
  21. Latt, S. A., and Loveday, K. S., Characterization of sister chromatid exchange in induction by 8-methoxypsoralen plus near UV light, Cytogenet. Cell Genet., 21: 184 (1978).CrossRefGoogle Scholar
  22. Natarajan, A. T., Van Buul, P. P. W., and Raposa, T., An evaluation of the use of peripheral blood lymphocyte systems for assessing cytological effects induced in vivo by chemical mutagens, in: “Mutagen-induced chromosome damage in man,” H. J. Evans and D. C. Lloyd, eds., University Press, Edinburgh, England (1978).Google Scholar
  23. Natarajan, A. T., Tates, A. D., Van Buul, P. P. W., Meijers, M., and De Vogel, N., Cytogenetic effects of mutagens/carcinogens after activation in a microsomal system in vitro. I. Induction of chromosome aberrations and sister chromatid exchanged by diethylnitrosamine (DEN) and dimethylnitrosamine (DMN) in CH0 cells in the presence of rat liver microsomes, Mutat. Res., 37: 83 (1976).Google Scholar
  24. Nevstad, N. P., Sister chromatid exchanges and chromosome aberrations induced in human lymphocytes by the cytostatic drug adriamycin in vivo and in vitro, Mutat. Res., 57: 253 (1978).Google Scholar
  25. Perry, P., and Evans, H. J., Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange, Nature, 258: 121 (1975).PubMedCrossRefGoogle Scholar
  26. Perry, P. E., and Searle, C. E., Induction of sister chromatid exchange in Chinese hamster cells by the hair dye constituents 2-nitro-p-phenylenediamine and 4-nitro-o-phenylenediamine, Mutat. Res., 56: 207 (1977).Google Scholar
  27. Perry, P. E., and Wolff, S., New Giemsa method for differential staining of sister chromatids, Nature, 261: 156 (1974).CrossRefGoogle Scholar
  28. Popescu, N. C., Amsburgh, S. C., and DiPaolo, J. A., Relationship of carcinogen induced sister chromatid exchange and neoplastic cell transformation, Int. J. Cancer, 28: 71 (1981).PubMedCrossRefGoogle Scholar
  29. Rudiger, H. W., Kohl, F., Mangeles, W., Von Wichert, P., Bartram, C. R., Wohler, W.,and Passarge, E., Benzpyrene induced sister chromatid exchanges in cultured human lymphocytes, Nature, 262: 290 (1976).PubMedCrossRefGoogle Scholar
  30. Schonwald, A. D., Bartram, C. R., and Rudiger, H. W., Benzpyrene induced sister chromatid exchanges in lymphocytes of patients with lung cancer, Hum. Genet., 36: 261 (1977).Google Scholar
  31. Schuler, C. F., and Latt, S. A., Sister chromatid exchange test in Chinese hamster cheek pouch mucosa, J. Dental Res., 578: 211 (1978).Google Scholar
  32. Siriani, S. R., and Huang, C. C., Comparison of induction of sister chromatid exchange, 8-azaguanine and ouabain-resistant mutants by cyclophosphamide, ifosfamide and 1-(pyridyl-3)-3,3-dimethylthriazene in Chinese hamster cells cultured in diffusion chambers in mice, Carcinogenesis, 1: 353 (1980).CrossRefGoogle Scholar
  33. Solomon, E., and Bobrow, M., Sister chromatid exchanges: A sensitive assay of agents damaging human chromosomes, Mutat. Res., 30: 273 (1975).Google Scholar
  34. Stetka, D. G., Minkler, J., and Carrano, A. V., Induction of longlived chromosome damage as manifested by sister chromatid exchange in lymphocytes of animals exposed to mitomycin-C, Mutat. Res., 51: 383 (1978).Google Scholar
  35. Stetka, D. G., and Wolff, S., Sister chromatid exchanges as an assay for genetic damage induced by mutagenic carcinogens, I. In vivo test for compounds requiring metabolic activation, Mutat. Res., 41: 333 (1976a).Google Scholar
  36. Stetka, D. G., and Wolff, S., Sister chromatid exchanges as an assay for genetic damage induced by mutagenic carcinogens, II. In vitro test for compounds requiring metabolic activation, Mutat. Res., 41: 343 (1976b).Google Scholar
  37. Swenson, D. H., Harbach, P. R., and Trzos, R. J., The relationship between alkylation of specific DNA bases and induction of sister chromatid exchange, Carcinogenesis, 1: 931 (1980).PubMedCrossRefGoogle Scholar
  38. Taylor, J. H., Sister chromatid exchanges in tritium labelled chromosomes, Genetics, 43: 515 (1958).PubMedGoogle Scholar
  39. Vogel, W., and Bauknecht, T., Differential chromatid staining by in vivo treatment as a mutagenicity test system, Nature, 260: 448 (1976).PubMedCrossRefGoogle Scholar
  40. Wolff, S., Chromosomal effects of mutagenic carcinogens and the nature of the lesions leading to sister chromatid exchange, in: “Mutagen-induced chromosome damge in man,” H. J. Evans and D. C. Lloyd, eds., University Press, Edinburgh, England (1978).Google Scholar
  41. Wolff, S., Bodycote, J., and Painter, R. B., Sister chromatid exchanges induced in Chinese hamster cells by UV irradiation of different stages of the cell cycle: The necessity for cells to pass through S, Mutat. Res., 25: 73 (1974).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Paul E. Perry
    • 1
  1. 1.MRC Clinical and Population Cytogenetics UnitWestern General HospitalEdinburghScotland

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