Cellular Specificity in DNA Damage, Repair, and Replication during Chronic Carcinogen Exposure

  • James G. Lewis
  • Mary A. Bedell
  • Kathyrn C. Billings
  • James A. Swenberg


In order to make rational use of human cells and tissues in the evaluation of risk from chemical or physical agents, it is crucial that a better understanding of the molecular events leading to malignant transformation be reached. It is of little use simply to replace studies on animal cells with human cells unless it is known which molecular processes and changes are relevant and should be quantified. In this paper, we present data in support of the hypothesis that the replication of DNA which contains promutagenic DNA damage is necessary for the initiation of carcinogenesis. If this hypothesis is true, and the relevant types of DNA damage can be identified and monitored in human cells, the possibility of quantitatively assessing risk using human cells will be greatly enhanced.


Cellular Specificity Cell Replication Methyl Methanesulfonate CsCl Gradient IARC Scientific Publication 
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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • James G. Lewis
    • 1
  • Mary A. Bedell
    • 1
    • 2
  • Kathyrn C. Billings
    • 1
    • 2
  • James A. Swenberg
    • 1
    • 2
  1. 1.Departments of PathologyDuke University Medical CenterDurhamUSA
  2. 2.Chemical Industry Institute of Toxicology, RTPUSA

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