In Vitro Studies on Chemical Carcinogenesis in BALB/c 3T3 Cells

  • Enrico Cortesi


During the last decade many in vitro systems have been developed as short-term tests for chemical, viral and physical carcinogenesis. A complete battery of such tests should include bacterial and mammalian mutagenesis, chromosome tests, DNA repair and cell transformation. These tests have been developed on the assumption that the oncogenic event is based on a genotoxic mechanism, the endpoint of which is detectable in a qualitative or quantitative way in each system.


Transformation Frequency Chemical Carcinogen Chemical Carcinogenesis Mouse Embryo Fibroblast Cell Chromosome Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    B. N. Ames, J. McCann, and E. Yamasaki, Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test, Mut. Res., 31: 347 (1975).Google Scholar
  2. 2.
    U. Saffiotti, P. J. Donovan, J. M. Rice, and E. Cortesi, Mutual synergism in Salmonella mutagenesis by aflatoxin B1, benzidine, benzo[a]pyrene and safrole, Teratogenesis, Carcinogenesis, and Mutagenesis (in press).Google Scholar
  3. 3.
    S. A. Aaronson and G. J. Todaro, Development of 3T3-like lines from BALB/c mouse embryo cultures: transformation susceptibility to SV 40, Science, 162: 1024 (1968).PubMedCrossRefGoogle Scholar
  4. 4.
    J. A. Di Paolo, K. Takano, and N. C. Popescu, Quantitation of chemically induced neoplastic transformation of BALB/3T3 cloned cell lines, Cancer Res., 32: 2686 (1970).Google Scholar
  5. 5.
    T. Kakunaga and J. Kamohora, Process of neoplastic transformation of cultured mammalian cell by chemical carcinogens, J. Cancer Assoc. Proc. Symp. 29th, p. 42 (1970).Google Scholar
  6. 6.
    T. Kakunaga and K. Miyashita, The involvement of DNA lesions and repair system in the cell transformation by chemical carcinogens, Symp. Cell Biol., Vol. 23: 95 (1972).Google Scholar
  7. 7.
    T. Kakunaga, A quantitative system for assay of maligant transformation by chemical carcinogens using a clone derived from BALB/3T3, Int. J. Cancer, 12: 463 (1973).PubMedCrossRefGoogle Scholar
  8. 8.
    T. Kakunaga and J. D. Crow, Cell variants showing differential susceptibility to ultraviolet light-induced-transformation, Science, 209: 505 (1980).PubMedCrossRefGoogle Scholar
  9. 9.
    A. Sivak et al., BALB/c-3T3 cells as target cells for chemically induced neoplastic transformation, Adv. Environ. Toxicol. (in press).Google Scholar
  10. 10.
    L. M. Schechtman and R. E. Kouri, Control of benzo(a)pyrene induced mammalian cell cytotoxicity, mutagenesis,and transformation by exogenous enzyme fractions, Progress in Genetic Toxicology: 307 (1977).Google Scholar
  11. 11.
    T. V. Zenser, M. B. Mattammal, H. J. Armbrecht, and B. B. David, Benzidine binding to nucleic acids mediated by the per-oxidative activity of prostaglandin endoperoxidase synthetase, Cancer Res., 40: 2839 (1980).PubMedGoogle Scholar
  12. 12.
    E. Cortesi, U. Saffiotti, P. J. Donovan, J. M. Rice, and T. Kakunaga, Dose–response studies on neoplastic transformation of BALB/c 3T3 clone A31–1–1 cells by aflatoxin Bi, benzidine, benzo(a)pyrene, 3–methylcholantrene and N–methyl–N’–nitro–Nnitrosoguanidine, Teratogenesis, Carcinogenesis, and Muta–genesis (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Enrico Cortesi
    • 1
  1. 1.Laboratory of Experimental Pathology, Division of Cancer Cause and PreventionNational Cancer InstituteFrederickUSA

Personalised recommendations