An Attempt to Determine the Origin of DNA Single-Strand Breaks Observed after Treatment of Mammalian Cells with Alkylating Agents

  • A. Abbondandolo
  • E. Dogliotti
  • P. H. M. Lohman
  • F. Berends


Upon exposure of DNA to alkylating agents a number of different lesions are produced, either directly or as a consequence of secondary modifications of the original alkylation. We have investigated the formation of one of these second lesions, single-strand DNA breaks (ssb), after treatment of cultured Chinese hamster ovary cells with four different ethylating agents: N-ethyl-N’-nitro-N-nitrosoguanidine (ENNG), N-ethyl-N-nitrosourea (ENU), ethylmethanesulfonate (EMS), and diethyl sulfate (DES).


Chinese Hamster Ovary Cell Alkaline Hydrolysis Lysis Time Repair Event Diethyl Sulfate 
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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • A. Abbondandolo
    • 1
  • E. Dogliotti
    • 2
  • P. H. M. Lohman
    • 3
  • F. Berends
    • 3
  1. 1.Istituto di Mutagenesi e Differenziamento CNRPisaItaly
  2. 2.lstituto Superiore di SanitàRomeItaly
  3. 3.Medical Biological Laboratory TNORijswijkThe Netherlands

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