The Strauss-Albertini Test for Direct Enumeration of Drug-Resistant Peripheral Blood Lymphocytes

  • G. H. S. Strauss

Abstract

Our objective in developing the Strauss-Albertini test was to provide a direct technique for assessing the in vivo incidence of mutant cells arising amongst circulating lymphocytes in animals and man. The test is an early attempt at direct estimation of genotoxic risk from environmental agents. My present intention is to review the development and primary applications of the test, update the method, and nurture interest in this seminal system. Except where indicated otherwise, I performed the experiments described here while a graduate student at the College of Medicine, University of Vermont (USA).

Keywords

Acute Lymphocytic Leukemia Labelling Index Variant Frequency Purine Analogue Free Nucleus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Albertini, R.J., 1979, Direct Mutagenicity testing with peripheral blood lymphocytes, in: ‘Banbury Report 2, Mammalian Cell Mutagenesis: The Maturation of Test Systems“, A.W. Hsie, J.P. O’Neill, and V.K. McElheny, eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor.Google Scholar
  2. Albertini, R.J., and De Mars, R., 1973, Detection and quantification of X-ray induced mutation in cultured diploid human fibroblasts, Mutation Res., 18: 199.PubMedCrossRefGoogle Scholar
  3. Albertini, R.J., and De Mars, R., 1974, Mosaicism of peripheral blood lymphocyte populations in females heterozygous for the Lesch-Nyhan mutation, Biochem. Genet., 11: 397.PubMedCrossRefGoogle Scholar
  4. Bender, R.A., and Young, R.C., 1978, Effects of cancer treatment on individual and generational genetics, Seminars in Oncology, 5: 46.Google Scholar
  5. Dancis, J., Berman, P.H., Jansen, V., and Balis, M.E., 1978, Absence of mosaicism in the lymphocyte in X-linked congenital hyperuricemia, Life Sci., 7: 587.CrossRefGoogle Scholar
  6. De Mars, R., 1971, Genetic studies of HGPRT deficiency and the Lesch-Nyhan syndrome with cultured human cells, Fed. Proc. 30: 944.Google Scholar
  7. De Mars, R., and Held, K., 1972, The spontaneous azaguanineresistant mutants of diploid human fibroblasts, Humangenetic, 16: 87.CrossRefGoogle Scholar
  8. McKeran, R.O., Howell, A., Andrews, R.M., Watts, R.W.E., and Arlett, C.F., 1974, Observations on the growth in vitro of myeloid progenitor cells and fibroblasts from hemizygotes and heterozygotes for “complete” and “partial” hypoxanthine-guanine phosphoribosyltransferase deficiency, and their relevance to the pathogenesis of brain damage in the Lesch-Nyhan syndrome, J. Neurol. Sci., 72: 183.CrossRefGoogle Scholar
  9. Strauss, G.H.S., in press, Direct mutagenicity testing: The development of a clonal assay to detect and quantitate mutant lymphocytes arising in vivo, in: “Banbury Report 13, Indicators of Genotoxic Exposures in Man and Animals,” V.K. McElheny, ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor.Google Scholar
  10. Strauss, G.A., and Albertini, R.J., 1977, 6 Thioguanine resistant lymphocytes in human peripheral blood, in: “Progress in Genetic Toxicology,” D. Scott, B.A. Bridges, and F.H. Sobels, eds., Elsevier/North-Holland, Amsterdam.Google Scholar
  11. Strauss, G.H., and Albertini, R.J., 1979a, Enumeration of 6-thioguanine resistant peripheral blood lymphocyte frequencies in man as a potential test for somatic cell mutation arising in vivo, Mutation Res., 41: 353.Google Scholar
  12. Strauss, G.H., and Albertini, R.J., 1979b, Longitudinal determination of 6-thioguanine resistant blood lymphocyte frequencies in individuals receiving 8-methoxypsoralen and long-wave ultraviolet light treatment (PUVA), Environ. Mutagen, 1: 152.Google Scholar
  13. Strauss, G.H., Albertini, R.J., and Allen, B.J., 1980, An enumerative assay of purine analogue resistant lymphocytes in women heterozygous for the Lesch-Nyhan mutation, Biochem. Genet. 18: 529.PubMedCrossRefGoogle Scholar
  14. Strauss, G.H., Albertini, R.J., Krusinski, P.A., and Baughman, R.D., 1979, 6-Thioguanine resistant peripheral blood lymphocytes in humans following psoralen, longwave ultraviolet light ( PUVA) therapy, J. Invest. Dermatol., 73: 211.PubMedCrossRefGoogle Scholar
  15. Subak-Sharpe, J.H., Burk, R.A., and Pitts, J.D., 1979, Metabolic co-operation between biochemically marked mammalian cells in tissue culture, J. Cell. Sci., 4: 353.Google Scholar
  16. Thilly, W.G., De Luca, J.G. Hoppe, I.V.H., and Penman, B.W., 1976, Mutation of human lymphoblasts by methylnitrosourea, Chem. Biol. Interact., 15: 33.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • G. H. S. Strauss
    • 1
  1. 1.Centre for Medical Research and MRC Cell Mutation UnitUniversity of SussexBrightonEngland

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