Electrophilic Reactivity as a Measure of Genotoxic Potency

  • Lars Ehrenberg
  • Siv Osterman-Golkar


The mutagenic effectiveness in forward mutation systems of a number of monofunctional alkylating agents with large differences in absolute reactivity and with different s-values (Swain and Scott, 1953) have been compared in the low-dose region of the dose-response curves. It was shown that the mutation frequency, R, at equal dose, D* (D is the time integral of the concentration of the alkylating agent, RX), was proportional to the second order rate constant, kn=2, for reaction with some nucleophilic center with a low nucleophilic strength (n=2 in the Swain-Scott scale, see Fig. 1) (Hussain and Ehrenberg, 1975; Osterman-Golkar, 1975; Ehrenberg, 1980; Hussain, 1981)


Alkylating Agent Sufficient Criterion Electrophilic Reactivity Acyl Halide Mutagenic Effectiveness 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ehrenberg, L., 1977, Aspects of statistical inference in testing for genetic toxicity, in: “Handbook of Mutagenicity Test Procedures,” B. Kilbey, et al., eds., Elsevier/North Holland, Amsterdam.Google Scholar
  2. Ehrenberg, L., 1980, Purposes and methods of comparing effects of radiation and chemicals, in: “Radiobiological Equivalents of Chemical Pollutants,” IAEA, Vienna.Google Scholar
  3. Ehrenberg, L., Moustacchi, E., and Osterman-Golkar, S., 1981, Dosimetry of genotoxic agents and dose-response relationships of their effects, Working paper for ICPEMC.Google Scholar
  4. Ehrenberg, L., and Osterman-Golkar, S., 1980, Alkylation of macromolecules for detecting mutagenic agents, Teratogenesis Carcinogenesis Mutagenesis, 1: 105.CrossRefGoogle Scholar
  5. Hussain, S.,1981, Doctoral Thesis, Stockholm (in preparation). Hussain, S., and Ehrenberg, L., 1975, Prophage inductive efficiency of alkylating agents and radiations, Int. J. Radiat. Biol., 27:355.CrossRefGoogle Scholar
  6. Miller, E. C., and Miller, J. A., 1971, The mutagenicity of chemical carcinogens: Correlations, problems and interpretations, in: “Chemical Mutagens,” vol. 1, A. Hollaender, ed., Plenum Press, New York.Google Scholar
  7. Osterman-Golkar, S., 1975, Studies on the reaction kinetics of biologically active electrophilic reagents as a basis for risk estimates, Doctoral Thesis, Stockholm.Google Scholar
  8. Segerbäck, D., and Ehrenberg, L., 1981, Alkylating properties of dichlorvos (DDVP), Acta Pharmacol. Toxicol., in press.Google Scholar
  9. Swain, C. G., and Scott, C. A., 1953, Quantitative correlation of relative rates. Comparison of hydroxide ion with other nucleophilic reagents towards alkyl halides, esters, epoxides and acyl halides, J. Am. Chem. Soc., 75: 141.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Lars Ehrenberg
    • 1
  • Siv Osterman-Golkar
    • 1
  1. 1.Department of Radiobiology, Wallenberg LaboratoryUniversity of StockholmStockholmSweden

Personalised recommendations