Parvoviral Probe for Assessing the Mutagenic Risk of Low Doses of Radiation and Chemicals Administered to Human Cells

  • J. J. Cornelis
  • C. Dinsart
  • Z. Z. Su
  • J. Rommelaere

Abstract

The marketing of an increasing number of chemicals makes it desirable to dispose of adequate tests for assessing their mutagenic/carcinogenic risk for man. The traditionally used animal tests are often long-lasting and very expensive, especially when the effect of low doses is sought. This led to the development of a battery of semi in vivo and in vitro tests using both prokaryotic and eukaryotic cells, which can be used routinely and serve as a guideline for the in vivo tests [1]. Cells collected from animals, including humans, can be cultured in vitro using appropriate nutritive media. Such cell cultures constitute a relatively well-defined system to analyze the toxic, mutagenic and transforming effect of exposure to environmental compounds. A widely used endpoint is the induction of chromatid and chromosome rearrangements. Visible karyotypic changes, however, do not necessarily accompany mutagenesis and do not provide an absolute indicator of the latter. Selective systems were thus developed, allowing the direct measurement of mutation induction in the genes of mammalian cells [2] or of viruses infecting those cells (see Introduction). The scope of this paper is to validate the use of the Hamster Osteolytic virus H-1, an autonomous parvovirus, for assessing the mutagenic risk associated with the exposure of human cells to low doses of radiations or chemicals.

Keywords

Simian Virus Xeroderma Pigmentosum Genotoxic Agent Mutation Assay Cell Pretreatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • J. J. Cornelis
    • 1
  • C. Dinsart
    • 1
  • Z. Z. Su
    • 1
  • J. Rommelaere
    • 1
  1. 1.Department of Molecular BiologyUniversité Libre de BruxellesRhode Saint GenèseBelgium

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