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Recombinant Protein Techniques

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Protein Analysis and Purification

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Abstract

The isolation and characterization of proteins parallels the major technical advances in protein chemistry. The first proteins that were characterized were those that could be isolated in large quantities. With the availability of more sensitive techniques, smaller and smaller quantities were required to produce detailed structural information. Newly evolving technologies, in conjunction with expression systems, have allowed the production of large amounts of any proteinacious factor. The isolation and structural characterization of proteins now depends only on the ingenuity of the investigator in devising an appropriate assay.

The time is approaching when a new protein will be designed on the drawing board, using predictive algorithms, and its subsequent synthesis, via cloning or peptide coupling, will offer new and interesting challenges for biochemists and molecular biologists.

—Gerald D. Fasman, 1989.

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Authors and Affiliations

  1. Massachusetts General Hospital, Jackson 715, G.I. Unit, 02114, Boston, MA, USA

    Ian M. Rosenberg

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  1. Ian M. Rosenberg
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© 1996 Springer Science+Business Media New York

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Rosenberg, I.M. (1996). Recombinant Protein Techniques. In: Protein Analysis and Purification. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4757-1108-0_11

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  • DOI: https://doi.org/10.1007/978-1-4757-1108-0_11

  • Publisher Name: Birkhäuser, Boston, MA

  • Print ISBN: 978-0-8176-3665-4

  • Online ISBN: 978-1-4757-1108-0

  • eBook Packages: Springer Book Archive

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