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Lipoxins pp 27-37 | Cite as

Phospholipase A2 Stimulated Release of Lipoxin B4 Formation from Endogenous Sources of Arachidonic Acid in Porcine Leukocytes

  • Patrick Y.-K. Wong

Summary

Incubation of an isoenzyme of phospholipase A2 (PLA2, isolated from snake venom) with porcine leukocytes resulted in the formation of several trihydroxytetraene- containing compounds which were derived from endogenous sources of arachidonic acid. The formation of these endogenous compounds was dose-dependent with an EC50 of approximately 1.25 x 10−8M. At this concentration of the isoenzyme and time (10 min) of explosure the cells remained viable as determined by the exclusion of trypan blue. The trihydroxytetraene compounds were purified by RP-HPLC and their identities were analyzed by U.V. spectrometry, GC/MS and by comparison with synthetic materials. The biologically derived compounds proved to be lipoxin B4 (5S,14R,15S-trihydroxy-6,10,12-trans-8-ciseicosatetraenoic acid) and its two structural isomers (8-trans-LXB4 and 14S-8-trans-LXB4). Results of the present study indicate that porcine leukocytes can generate lipoxin 84 and its isomers from endogenous sources of arachidonic acid. Moreover, they suggest that certain PLA2 isoenzymes may stimulate the formation of lipoxins and related compounds.

Keywords

Arachidonic Acid Snake Venom Endogenous Source Eicosatetraenoic Acid Endogenous Arachidonic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

15-HPETE

15-hydroperoxyeicosatetraenoic acid

15-HPETE

15-hydroperoxyeicosatetraenoic acid

AA

Arachidonic Acid

EPA

Eicosapentaenoic acid

RP-HPLC

Reverse Phase-High Performance Liquid Chromatography

GC/MS

Gas Chromatography/Mass Spectrometry

LXB4

Lipoxin 84:(5S,14R,15S)-5,14,15- trihydroxy-6,10,12-trans-8-cis- eicosatetraenoic acid

8-trans-LXB4

(5S,14R,15S)-5,14,15-trihydroxy-6,8,10,12-trans eicosatetraenoic acid

14S-8-trans LXB4

(5S,145,15S)-5,14,15-trihydroxy-6,8,10,12-trans eicosatetraenoic acid

PLA2

Phospholipase A2

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Patrick Y.-K. Wong
    • 1
  1. 1.Department of PharmacologyNew York Medical CollegeValhallaUSA

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