Kinins—II pp 59-65 | Cite as

An Approach to the Chemical Quantitation of Kinins for the Assay of Kininogenases

  • B. T. Khouw
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


A method was developed for the rapid isolation and identification of kallidin produced by hog pancreatic kallikrein on a partially purified bovine plasma kininogen preparation. By means of a reversed phase chromatography on a C18-silica gel column, kallidin was rapidly concentrated from the reaction mixture, and subsequently resolved on a thin-layer plate of silica gel 60. Fluorescamine was used to locate the kinin. Efforts to elute the kinin from the TLC plates for quantitation were not successful.


Reversed Phase Chromatography Chemical Quantitation Fluorescent Spot Rapid Isolation Urinary Kallikrein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Gornall, A.G., C.J. Bardawill and M.M. David, 1949, Determination of serum proteins by means of biuret reaction, J. Biol. Chem., 177: 751.PubMedGoogle Scholar
  2. Mares-Guia, M., et al., A comparative study of urinary kallikrein isolated from man and the rat, 1975 In: Chemistry and Biology of the Kallikrein-Kinin System in Health and Disease, eds., J.J. Pisano and K.F. Austen (Fogarty Internat. Center Proc. No. 27, U.S. Gov. Printing Office, Washington ) p. 97.Google Scholar
  3. Mendez, E. and C.Y. Lai, 1975, Reaction of peptides with fluorescamine on paper after chromatography or electrophoresis, Analyt. Biochem. 65: 281.Google Scholar
  4. Komiya, M., H. Kato and T. Suzuki, 1974, Bovine plasma kininogen. III. Structural comparison of high molecular weight and low molecular weight kininogens. J. Biochem. (Tokyo), 76: 833.Google Scholar
  5. Udenfriend, S., et al., 1972, Applications of fluorescamine, a new reagent for assay of amino acids, peptides, proteins and other primary amines in the picomole range. Science, 178: 871.PubMedCrossRefGoogle Scholar
  6. Webster, M.E. and E.S. Prado, Glandular kallikreins from horse and human urine and from hog pancreas, 1970, In: Methods in Enzymology, Vol. 14, Eds., G.E. Perlmann and L. Lorand ( Academic Press, New York ) p. 681.Google Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • B. T. Khouw
    • 1
  1. 1.Research CentreCanada Packers, Ltd.TorontoCanada

Personalised recommendations