Kinins—II pp 375-391 | Cite as

How do Kinins Affect Vascular Tone?

  • Una S. Ryan
  • James W. Ryan
  • Cecil Whitaker
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


Because kinins affect vascular tone, it is assumed that kinins act directly on smooth muscle. However, a direct difficult to conceive. Vessels containing smooth muscle are lined by a continuous endothelium with tight junctions. In addition, kinins act on endothelial cells to cause the release of prostaglandin-related substances; possibly through receptors. Furthermore, endothelial cells have a great capacity for hydrolyzing kinins to inactive products. Hence, even invoking active transport, less than 1% of kinins might be expected to reach the first layer of smooth muscle cells. However, kinins may not act directly on smooth muscle as endothelial cells and smooth muscle cells form. specialized cell contacts. Myoendothelial junctions occur, and we have shown, in pulmonary arterioles, that smooth muscle cells send large numbers of projections into the cytoplasm of the endothelial cells. In addition, smooth muscle cells attach directly to the abluminal surface of endothelial cells, as do pericytes. Thus, there is a morphologic basis by which kinins can affect tone of smooth muscle without acting directly on smooth muscle cells.


Endothelial Cell Angiotensin Converting Enzyme Pulmonary Vascular Resistance Vascular Tone High Affinity Binding Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bito, L.A. and R.A. Baroody, 1975. Inhibition of pulmonary prostaglandin metabolism by inhibitors of prostaglandin biotransport (probenecid and bromcresol green), Prostaglandins, 10: 633Google Scholar
  2. Blumberg, A.L., S.E. Denny, G.R. Marshall and P. Needleman, 1977. Blood vessel-hormone interactions: Angiotensin, bradykinin, and prostaglandins, Amer. J. Physiol., 323: H305.Google Scholar
  3. Buonassisi, V. and J.C. Venter, 1976. Hormone and neurotransmitter receptors in an established vascular endothelial cell line, Proc. Natl. Acad. Sci., 73: 1612.PubMedCrossRefGoogle Scholar
  4. Chin, A.T., J.W. Ryan, U.S. Ryan and F.E. Dorer, 1975. A sensitive radiochemical assay for angiotensin converting enzyme (kininase II), Biochem. J., 149: 297.Google Scholar
  5. Eiling, T.E. and M.W. Anderson, 1976. Studies on the biosynthesis, metabolism, and transport of prostaglandine by the lung, Agents and Actions, 6: 543.CrossRefGoogle Scholar
  6. Farquhar, M.G. and G.E. Palade, 1963. Junctional complexes in various epithelia, J. Cell Biol., 17: 375Google Scholar
  7. Fawcett, D.W., 1961. Intercellular bridges, Exp. Cell Res, Suppl., 8: 174.Google Scholar
  8. Ferreira, S.H. and J.R. Vane, 1967. The disappearance of bradykinin and eledoisin in the circulation and vascular beds of the cat, Br. J. Pharmac., 30: 417.Google Scholar
  9. Friend, D.S. and N.B. Gilula, 1972. Variations in tight and gap junctions in mammalian tissues, J. Cell Biol., 53: 758Google Scholar
  10. Gimbrone, M.A. Jr. and R.W. Alexander, 1975. Angiotensin II stimulation of prostaglandin production in cultured human vascular endothalium, Science, 189: 219.PubMedCrossRefGoogle Scholar
  11. Cimbrone, M.A. and R.W. Alexander, 1977. Insulin receptors in cultured human vascular endothelial cells, Circulation, 56: 209.Google Scholar
  12. Habliston, D.L., C. Whitaker, M.A. Hart, U.S. Ryan and J.W. Ryan, (in press), Isolation and culture of endothelial cells from the lungs of small animals, Amer. Rev. Resp. Dis.Google Scholar
  13. Huxley, H.E., 1963. Electron microscope studies on the structure of natural and synthetic protein filaments from striated muscle, J. Mol. Biol., 3: 281.CrossRefGoogle Scholar
  14. Ives, H.E., R.E. Galardy and J.D. Jamieson, 1976. Isolation of functional aortic smooth muscle cells, J. Cell Biol., 70: 328a.Google Scholar
  15. Larsen W.J., 1977. Structùral diversity of gap junctions. A Review, Tissue Cell, 9: 373.PubMedCrossRefGoogle Scholar
  16. Pearson, J.D., J.S. Carleton, A. Hutchings and J.L. Gordon, 1978. Uptake and metabolism of adenosine by pig aortic endothelial and smooth-muscle cells in culture, Biochem. J., 170: 265.PubMedGoogle Scholar
  17. Piper, P.J. and J.R. Vane, 1971. The release of prostaglandins from lung and other tissues, Ann. N.Y. Acad. Sci., 180: 363.PubMedCrossRefGoogle Scholar
  18. Revel, J.P. and M.J. Karnovsky, 1967. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol., 33: C7.PubMedCrossRefGoogle Scholar
  19. Rhodin, J.A.G., 1967. The ultrastructure of mammalian arterioles and precapillary sphincters. J. Ultrastruct. Res., 18: 181.PubMedCrossRefGoogle Scholar
  20. Rhodin, J.A.G., 1968. Ultrastructure of mammalian venous capillaries venules, and small collecting veins. J. Ultrastruct. Res. 25: 452.PubMedCrossRefGoogle Scholar
  21. Rhodin, J.A.G., 1978. Microscopic anatomy of the pulmonary vascular bed in the cat lung. Microvasc. Res., 15: 169.PubMedCrossRefGoogle Scholar
  22. Rowe, G.G., S. Afonson, C.A. Castillo, F. Lioy, J.E. Lugo and C.W. Crumpton, 1963. The systemic and coronary hemodynamic effects of synthetic bradykinin. Am. Heart J., 65: 656.PubMedCrossRefGoogle Scholar
  23. Ryan, James W., A. Chung,,L.C. Martin and U.S. Ryan, 1978b. New substrates for the radioassay of angiotensin converting enzyme of endothelial cells in culture, Tissue Cell, 10: 555.Google Scholar
  24. Ryan, J.W., A.R. Day, U.S.” Ryan, A. Chung, D.I. Marlborough and F.E. Dorer, 1976a. Localization of angiotensin converting enzyme (kininase II) I. Preparation of antibody-heme-octa-peptide conjugates. Tissue Cell, 8: 111.PubMedCrossRefGoogle Scholar
  25. Ryan, ‘J.W., J. Roblero and J.M. Stewart, 1968. Inactivation of bradykinin in the pulmonary circulation. Biochem. J., 110: 795.Google Scholar
  26. Ryan, J.W., J. Roblero and J.M. Stewart. Inactivation of bradykinin in rat lung., 1970, In: Adv. Exp. Med. Biol. (Bradykinin and related Kinins), Vol., 8, eds. N. Back, F. Sicuteri and M. Kocha e Silva, (Plenum Press, New York) p. 263Google Scholar
  27. Ryan, J.W. and U.S. Ryan. Ryan,“1977. Pulmonary endothelial cells. Fed. Proc., 36: 2683.Google Scholar
  28. Ryan, J.W., U.S. Ryan, D.H. Habliston and L.C. Martin, 1978c. (in 1978c. Synthesis ofprostaglandins by pulmonary endothelial cells. Trans. Assoc.:Amer. Physns4, 91, 343.Google Scholar
  29. Ryan, J.W. and U. Smith, 1971. Metabolism of adenosine-Y -mono-phosphate during circulation through the lungs. Trans. Assoc. Physns., 84: 297.Google Scholar
  30. Ryan, J.W. and U. Smith. The metabolism of angiotensin i by endothelial cells, 1973. In: Protides of the Biological Fluids, Vol. 20, ed. H. Peeters, (Pergamon Press, Oxford, England ), p. 379.Google Scholar
  31. Ryan, U.S., E. Clements, D. Habliston and J.W. Ryan, 1978a. Isolation and culture of pulmonary artery endothelial cells. Tissue Cell, 10: 535.PubMedGoogle Scholar
  32. Ryan, U.S., J.W. Ryan and A. Chiu, 1976c. Kininase II (angiotensin converting enzyme) and endothelial cells in culture. In: (Kinins),Vol. 70, eds. F. Sicuteri, N. Back, and G.L. Haberland, ( Plenum Press, New York ), p. 217.Google Scholar
  33. Ryan, U.S., J.W. Ryan, D.L. Habliston and G.A. Pena, (this volume). Endothelial cells and components of the kallikrein-kinin system, 1979, p. 313.Google Scholar
  34. Ryan, U.S., J.W. Ryan, C. Whitaker and A. Chiu, 1976b, Localization of angiotensin converting enzyme (kininase II). II. Immunocytochemistry and immunofluorescence, Tissue Cell, 8: 125Google Scholar
  35. Schneeberger, E.E. and M.J. Karnovsky, 1976. Ultrastructure of intercellular junctions in freeze-fractured alveolar capillary membranes of mouse lung. Circ. Res., 38: 404.PubMedCrossRefGoogle Scholar
  36. Smith, U. and J.W. Ryan, 1970. An electron microscopic study of the vascular endothelium as a site for bradykinin and ATP inactivation in rat lung. In: Adv. Exp. Med. Biol., Vol. 8, eds. N. Back, F. Sicuteri and M. Roche e Silva, ( Plenum Press, New York ), p. 249.Google Scholar
  37. Smith, U. and J.W. Ryan, 1973. Electron microscopy of endothelial and epithelial components of the lungs: Correlations of structure and function. Fed. Proc., 32: 1957.PubMedGoogle Scholar
  38. Wong, P.Y,-K., D.A. Terragno, N.A. Terragno and J.C. McGiff, 1977. Dual effects of bradykinin on prostaglandin metabolism: Relationship to the dissimilar vascular actions of kinins, Prostaglandins, 13: 1113.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Una S. Ryan
    • 1
  • James W. Ryan
    • 1
  • Cecil Whitaker
    • 1
  1. 1.Department of MedicineUniversity of Miami School of MedicineMiamiUSA

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