Kinins—II pp 305-312 | Cite as

A Simple, Large Scale Process for Purifying Human Urinary Kallikrein, Based on Reverse Osmosis and Ammonium Sulfate Precipitation

  • Amintas F. S. Figueiredo
  • Marcos Mares-Guia
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


A simple, large scale process for the purification of human urinary kallikrein is described which is based upon concentration by reverse osmosis, ammonium sulfate precipitation, and gel filtration on a column of Sephadex-G-150. The yield in protein is higher than any reported in the literature to this date; the purified enzyme seems to be identical to that reported by Figueiredo and Mares-Guia at the Klein-Symposium in Paris, 1978, based on the procedure of Hial, et al., (1974).


Ammonium Sulfate Reverse Osmosis Human Urinary Amino Acid Analysis Ammonium Sulfate Precipitation 
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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Amintas F. S. Figueiredo
    • 1
  • Marcos Mares-Guia
    • 1
  1. 1.Depto. De Bioquimica, Inst. Cien. Biol.Universidade Federal De Minas Gerais30.000 Belo HorizonteBrazil

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