Abstract
A simple, large scale process for the purification of human urinary kallikrein is described which is based upon concentration by reverse osmosis, ammonium sulfate precipitation, and gel filtration on a column of Sephadex-G-150. The yield in protein is higher than any reported in the literature to this date; the purified enzyme seems to be identical to that reported by Figueiredo and Mares-Guia at the Klein-Symposium in Paris, 1978, based on the procedure of Hial, et al., (1974).
This work was supported by FINEP.
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Figueiredo, A.F.S., Mares-Guia, M. (1979). A Simple, Large Scale Process for Purifying Human Urinary Kallikrein, Based on Reverse Osmosis and Ammonium Sulfate Precipitation. In: Fujii, S., Moriya, H., Suzuki, T. (eds) Kinins—II. Advances in Experimental Medicine and Biology, vol 120. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-0926-1_29
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DOI: https://doi.org/10.1007/978-1-4757-0926-1_29
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