Kinins—II pp 273-290 | Cite as

Purification and Characterization of Hog Pancreatic Kallikrein I, II and III

  • Heizo Kira
  • Seiji Hiraku
  • Hiroshi Terashima
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


Three forms of kallikrein [EC] were purified from hog pancreas powder by affinity chromatography on bovine lung trypsin inhibitor-Sepharose column and DEAE-Sephadex A-50 column chromatography. Each form of kallikrein was tentatively. named II, III and I, in the order of elution on DEAE-Sephadex A-50 column. Final yields of I, II and III, per kg of the starting material, were 6.7, 7.6 and 1.9 mg, respectively. Kallikrein I and II were crystallized.


Soybean Trypsin Inhibitor Ammonium Acetate Buffer Vasodilator Activity Urea Treatment Chloromethyl Ketone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Heizo Kira
    • 1
  • Seiji Hiraku
    • 1
  • Hiroshi Terashima
    • 1
  1. 1.Research InstituteOno Pharmaceutical Co., Ltd.Sakurai, Shimamoto-cho, Mishima-gun, Osaka 618Japan

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