Kinins—II pp 203-211 | Cite as

Assay of Angiotensin I by Fluorescence Polarization Method

  • Hiroshi Maeda
  • Mahito Nakayama
  • Daisuke Iwaoka
  • Tatsuo Sato
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

Fluorescence polarization technique was applied for the assay of angiotensin I (Al) in human plasma. In this assay system, fluorescein labeled Al (F-Al), which retained the original antigenicity, and antibody to Al was allowed to interact in a cuvette in the instruments yielding an increase in the fluorescence polarization (P) value. Non-labeled Al in the sample blocked the binding of F-Al to the antibody resulting lower P value. Log of antigen concentration and P value was found to exhibit reverse linear proportionality between 0.05 ng to 2 ng/ml of antigen (Al) concentration. The present method was compared with standard radio-immunoassay method and the result showed that data were compatible with each other. The calculation of P value is automated and three cavity filter and optics of the instrument gave reliable results. The method is fast (<2 min), sensitive (<10 picomole/ml) and simple (no separation step before readout of the results).

Keywords

Renin Activity Phenyl Methyl Sulfonyl Fluoride Fluorescence Polarization Anode Voltage Phenyl Methyl Sulfonyl Fluoride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Hiroshi Maeda
    • 1
  • Mahito Nakayama
    • 2
  • Daisuke Iwaoka
    • 2
  • Tatsuo Sato
    • 2
  1. 1.Departments of MicrobiologyKumamoto University Medical SchoolKumamotoJapan 860
  2. 2.Internal MedicineKumamoto University Medical SchoolKumamotoJapan 860

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