Kinins—II pp 165-172 | Cite as

Determination of Hageman Factor (HG, Factor XII) and Plasma Prekallikrein (Fletcher Factor) by Radioimmunoassays

  • Hidehiko Saito
Part of the Advances in Experimental Medicine and Biology book series (AEMB)


The titers of components of the plasma kallikrein-kinin system have been measured conventionally by their biological functions. The functional assays are, however, antagonized by the presence of inhibitors and/or the absence of potentiators in test samples. Immunologic assays obviate these difficulties. We have developed specific, sensitive and reproducible radioimmunoassays (RIA) for HF and prekallikrein, and have applied these assays to some clinical conditions. Normal pooled human plasma contained approximately 40 μg of HF and 50 μg of prekallikrein per ml. RIA were able to measure concentrations of HF and prekallikrein as low as 0.1% and 0.3% that of normal pooled plasma respectively. A good correlation existed between titers measured by clotting and radioimmunoassays among 40 normal subjects (correlation co-efficient = 0.82 for HF and 0.71 for prekallikrein). There was no significant difference between the levels of HF and prekallikrein in plasma and those in serum. Both HF and prekallikrein were significantly reduced in plasmas of patients with advanced liver cirrhosis or disseminated intravascular coagulation (DIC) and in cord serums, but they were normal in plasmas obtained after strenuous physical exercise and in plasmas of patients under treatment with warfarin.


Disseminate Intravascular Coagulation Disseminate Intravascular Coagulation Strenuous Physical Exercise Cord Serum High Molecular Weight Kininogen 
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  1. Amundsen, E., L. Svendsen, A.M. Vennerd, and K. Laake. 1977. Determination of plasma kallikrein with a new chromogenic tripeptide derivative. In: Chemistry and Biology of KallikreinKinin System in Health and Disease. Pisano, J.J. and Austen, K.F. (eds.) (DREW Pub. No. 76–791, Washington, D.C. p. 215 ).Google Scholar
  2. Bagdassarian, A., B. Lahiri, R.C. Talamo, P. Wong and R.W. Colman. 1974. Immunochemical Studies of Plasma Kallikren. J. Clin. Invest. 54: 1444.Google Scholar
  3. Colman, R.W., J.W. Mason and S. Sherry. 1969. The kallikreinogenkallikrein enzyme system of human plasma. Assay of components and observations in disease states. Ann. Intern. Med. 71: 763.Google Scholar
  4. Donaldson, V.H., J. Kleniewski, H. Saito and J.K. Sayed. 1977. Prekallikrein deficiency in a kindred with kininogen deficiency and Fitzgerald trait clotting defect. Evidence that high molecular weight kininogen and prekallikrein exist as a complex in normal human plasma. J. Clin. Invest. 60: 571.Google Scholar
  5. Hathaway, W.E., L.P. Belhasen and H.S. Hathaway. 1965. Evidence for a new plasma thromboplastin factor I. Case report, coagulation studies and physico-chemical properties. Blood. 26: 521.Google Scholar
  6. Kluft, C. 1978. Determination of prekallikrein in human plasma: Optimal conditions for activating prekallikrein. J. Lab. Clin. Med. 91: 83.Google Scholar
  7. Kurkcuoglu, N. and A.E. Mc Elfresh. 1960. The Hageman factor: Determination of its concentration during the neonatal period and presentation of a case of Hageman factor deficiency. J. Pediatr. 57: 61.Google Scholar
  8. Margolis, J. 1958. Activation of plasma by contact with glass: Evidence for a common reaction which releases plasma kinin and initiates coagulation. J. Physiol. ( Lond. ) 144: 1.Google Scholar
  9. Mason, J.W., U.R. Kleeberg, P. Dolan and R.W. Colman, 1970. Plasma kallikrein and Hageman factor in gram-negative bacteremia. Ann. Intern. Med. 73: 545.Google Scholar
  10. Ratnoff, O.D. and J.E. Colopy. 1955. A familial hemorrhagic trait associated with a deficiency of a clot-promoting fraction of plasma. J. Clin. Invest. 34: 602.Google Scholar
  11. Saito, H., O.D. Ratnoff and J. Pensky. 1976a. Radioimmunoassay of human Hageman factor (factor XII). J. Lab. Clin. Med. 88: 506.Google Scholar
  12. Saito, H., G.H. Goldsmith and R. Waldmann. 1976b. Fitzgerald factor (high molecular weight kininogen) clotting activity in human plasma in health and disease, and in various animal plasmas. Blood. 48: 941.PubMedGoogle Scholar
  13. Saito, H. and O.D. Ratnoff. 1977. Molecular heterogeneity of human plasma prekallikrein deficiency (Fletcher trait). Clin. Res. 25: 347A.Google Scholar
  14. Saito, H., M.C. Poon, W. Vicic, G.H. Goldsmith and J.E. Menitove. 1978a. Human plasma prekallikrein (Fletcher factor) clotting activity and antigen in health and disease. J. Lab. Clin. Med. 92: 84.Google Scholar
  15. Saito, H., J.G. Scott, H.Z. Movat and S.J. Scialla. 1978b. CRM+ Hageman trait. Blood. 52: (supplement 1): 194.Google Scholar
  16. Wuepper, K.D. 1973. Prekallikrein deficiency in man. J. Exp. Med. 138: 1345.Google Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • Hidehiko Saito
    • 1
  1. 1.Department of MedicineCase Western Reserve University School of Medicine and University Hospitals of ClevelandClevelandUSA

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