Abstract
A method was developed to measure kininase activity in human urine. The method consists of dialysis of human centrifuged urine sample against phosphate buffer and partial fractionation of A-50 Sephadex column. The enzymatic property of urinary kininase, which destroys bradykinin when incubated, is estimated from its effect on a definite amount of bradykinin, using rat uterus.
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References
Erdös, E.G. and Yang, H.Y.T. (1970) Kíninases - in: Hdb of Exp. Pharmacol., Vol. XXV, Ed. by Erdös, E.G., Springer Verlag, N.Y., p. 289.
Greco, A., Porcelli G., Di Jorio, M., Ranieri M., D’Acquarica, L. and Ranalli L., Altered urinary Excretion of human kininase activity in hypertension; same issue
Greco, A., Porcelli, G., Rebuzzi, A.G., Di Jorio, M., Ranieri, M. and Ranalli L., Altered urinary excretion of human kininase activity in acute myocardial infarction; same issue
Levine, B.W., Talamo, R.C. and Kazemi, H. (1973), Action and metabo- lism of bradykinin in the dog lung, J.Appl.Physiol., 34, 821
Porcelli, G., Marini-Bettòlo, G.B., Di Jorio, M., Ranieri, M., Ranallí, L., D’Acquarica, L., Preliminary purification and biochemical characterization of human urinary kininase; same issue.
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© 1979 Springer Science+Business Media New York
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Porcelli, G., Di Jorio, M., Ranieri, M., Ranalli, L., D’Acquarica, L. (1979). Method for Measurement of Human Urinary Kininase Activity. In: Fujii, S., Moriya, H., Suzuki, T. (eds) Kinins—II. Advances in Experimental Medicine and Biology, vol 120. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-0926-1_14
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DOI: https://doi.org/10.1007/978-1-4757-0926-1_14
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