Excitation Energy Transfer in Purple Photosynthetic Bacteria: Analysis by the Time-Resolved Fluorescence Spectroscopy

  • Mamoru Mimuro
  • Keizo Shimada
  • Naoto Tamai
  • Iwao Yamazaki
Part of the FEMS Symposium book series (FEMSS)


Absorption of light is the primary event to drive photosynthesis in photosynthetic bacteria. The light energy is then transferred among antenna pigments and finally delivered to reaction center (RC) where the photochemical charge separation takes place. The RC polypeptides are known to be highly conserved through many kinds of photosynthetic organisms [1]; RC II in higher plants, “quinone type RC”, is similar to RC of purple photosynthetic bacteria, whereas RC I, “Fe-S type RC”, to that of (strictly) anaerobic green bacteria (Chlorobium limicola or Heliobacterium chlorurn). On the other hand, the polypeptides of antenna pigment protein complex are divergent; only a partial similarity is suggested [2]. Physical basis to sustain the function of pigment protein complex, however, might be similar to each other, which is not clearly elucidated.


Reaction Center Transfer Time Decay Kinetic Pigment System Fluorescence Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





reaction center


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  1. 1.
    Blankenship, R.E., Brune, D.C., Freeman, J.M., King, G.H., McManus, J,H., Nozawa, T., Trost, J.T. and Wittmershaus, B.P. (1988) in Green Photosynthetic Bacteria, (Olson, J.M., Orrnerod, J.G., Amesz, J., Stackebrandt, E. and Truper, H.G. eds.), pp. 57–68, Plenum Press, New York.CrossRefGoogle Scholar
  2. 2.
    Zuber, H. (1987) in The Light Reactions (Barber, J. ed.), pp. 197–259, Elsevier, Amsterdam.Google Scholar
  3. 3.
    van Grondelle, R. (1985) Biochim. Biophys. Acta 811, 147–195.CrossRefGoogle Scholar
  4. 4.
    Kramer, H.J.M., Pennoyer, J.D., van Grondelle, R., Westerhuis, W.H.J., Niederman, R.A. and Amesz, J. (1984) Biochim. Biophys. Acta 767, 335–344.CrossRefGoogle Scholar
  5. 5.
    van Grondelle, R. and Sundstrom, V. (1988) in Photosynthetic Light-Harvesting Systems; Organization and Function, (Scheer, H. and Schneider, W. eds.), pp. 403–438, Walter de Gryuter, Berlin.Google Scholar
  6. 6.
    Sundstrom, V., van Grondelle, R., Bergstrom, H., Akesson, E. and Gillbro, T. (1986) Biochim. Biophys. Acta 851, 431–446.CrossRefGoogle Scholar
  7. 7.
    van Grondelle, R., Hunter, C.N., Bakker, J.G.C. and Kramer, H.J.M. (1983) Biochim. Biophys. Acta 723, 30–36.CrossRefGoogle Scholar
  8. 8.
    Borisov, A.Yu., Gadonas, R.A., Danielius, R.V., Piskarskas, A.S. and Razjivin, A.P. (1982) FEBS Lett., 138, 25–28.CrossRefGoogle Scholar
  9. 9.
    Hunter, C. N., Kramer, H.J.M. and van Grondelle, R. (1985) Biochim. Biophys. Acta 807, 44–51.CrossRefGoogle Scholar
  10. 10.
    Vos, M., van Dorssen, R.J., Amesz, J., van Grondelle, R. and Hunter, C.N. (1988) Biochim. Biophys. Acta 933, 132–140.CrossRefGoogle Scholar
  11. 11.
    Mimuro, M. (1988) in Photosynthetic Light-Harvesting Systems; Organization and Function, (Scheer, H. and Schneider, W. eds.), pp. 589–600, Walter de Gruyter, Berlin.Google Scholar
  12. 12.
    General Discussion in Chlorophyll Organization and Energy Transfer in Photosynthesis (1978) Ciba-Foundation Symposium 61, pp. 341–364, Excerpta Medica, Amsterdam.Google Scholar
  13. 13.
    Beechem, J.M. and Brand, L. (1986) Photochem. Photobiol., 44, 323–329.PubMedCrossRefGoogle Scholar
  14. 14.
    Harashima, K., Shiba, T. and Murata, N. (1989) in Aerobic Phototsynthetic Bacteria, Japan Scientific Societies Press, Tokyo.Google Scholar
  15. 15.
    Shimada, K., Hayashi, H. and Tasumi, M. (1985) Arch. Microbiol., 143, 244–24 7.Google Scholar
  16. 16.
    Yamazaki, I., Mimuro, M., Murao, T., Yamazaki, T., Yoshihara, K. and Fujita, Y. (1984) Photochem. Photobiol. 39, 233–240.CrossRefGoogle Scholar
  17. 17.
    Mimuro, M., Yamazaki, I., Itoh, S., Tamai, N. and Satoh, K. (1988) Biochim. Biophys. Acta 933, 478–486.CrossRefGoogle Scholar
  18. 18.
    Shimada, K., Mimuro, M., Tamai, N. and Yamazaki, I. (1989) Biochim. Biophys. Acta, 975, 72–79.CrossRefGoogle Scholar
  19. 19.
    Itoh, M., Matsuura, K., Shimada, K. and Satoh, T. (1988) Biochim. Biophys. Acta, 936, 332–338.CrossRefGoogle Scholar
  20. 20.
    Kramer, H.J.M., van Grondelle, R., Hunter, C.N., Westerhuis, W.H.J. and Amesz, J. (1984) Biochim. Biophys. Acta 765, 156–165.CrossRefGoogle Scholar
  21. 21.
    Sebban, P., Jolchine, G. and Moya, I. (1984) Photochem. Photobiol. 39, 247253.Google Scholar
  22. 22.
    Borisov, A.Yu., Freiberg, A., Godik, V.I. Rebane, K.K. and Timpmann, K.E. (1985) Biochim. Biophys. Acta 807, 221–229.CrossRefGoogle Scholar
  23. 23.
    Freiberg, A., Godik, V.I., Pullertis, T. and Timpman, K. (1989) Biochim. Biophys. Acta, 973, 93–104.CrossRefGoogle Scholar
  24. 24.
    Stepanov, B.I. (1957) Dokl. Acad. Nauk USSR 112, 839–841.Google Scholar
  25. 25.
    Goedheer, J.C. (1972) Biochim. Biophys. Acta, 275, 169–176.Google Scholar
  26. 26.
    Sebban, P., Robert, B. and Jolchine, G. (1985) Photochem. Photobiol., 42, 573–578.CrossRefGoogle Scholar
  27. 27.
    Zankel, K.L. (1978) in The Photosynthetic Bacteria (Clayton, R.K. and Sistrom, W.R., eds.), pp. 341–347, Plenum Press, New York.Google Scholar
  28. 28.
    Bergstrom, H., Westerhuis, W.H.J., Sundstrom, V., van Grondelle, R., Nieder-man, R.A. and Gillbro, T. (1988) FEBS Letters, 233, 12–16.CrossRefGoogle Scholar
  29. 29.
    Hunter, C.N., van Grondelle, R., and Olsen, J.D. (1989) Trends Biochem. Sci., 14, 72–76.PubMedCrossRefGoogle Scholar
  30. 30.
    Matsuura, K. and Shimada, K. (1986) Biochim. Biophys. Acta 852, 9–18.Google Scholar
  31. 31.
    Hayashi, II., Shimada, K., Tasumi, M. Nozawa, T. and Hatano, M. (1986) Photobiochem. Photobiophys., 10, 223–231.Google Scholar
  32. 32.
    Ke, B. and Schuvalov, V.A. (1987) in The Light Reaction, (Barber, J. ed.), pp. 31–93, Elsevier, Amsterdam.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Mamoru Mimuro
    • 1
  • Keizo Shimada
    • 2
  • Naoto Tamai
    • 3
  • Iwao Yamazaki
    • 3
  1. 1.National Institute for Basic BiologyAichi 444Japan
  2. 2.Department of Biology, Faculty of ScienceTokyo Metropolitan UniversityTokyo 164Japan
  3. 3.Institute for Molecular ScienceAichi 444Japan

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