Spectroscopic and Structural Studies of Crystallized Reaction Centres from Wild Type Rhodobacter Sphaeroides Y
Spectroscopic studies on reaction centre (RC) crystals are a powerful tool to answer a number of questions. First one may ask if the pigments have been altered or not during the crystallization process. Second, photoinduced electron tranfer may be studied in the crystalline state and kinetics may be compared to those observed in solution. Finally, when the three-dimensionnal structure of the reaction center has been solved, the polarized absorption spectra may be analyzed to assign the individual absorption bands to oriented transition moments of each ohromophore [1,2].
KeywordsReaction Centre Charge Recombination Linear Dichroism Secondary Quinone Measuring Light Intensity
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- Ducruix, A., Arnoux, B. and Reiss-Husson, F. (1988); in: The Photosynthetic Bacterial Reaction Center: Structure and Dynamics (Breton, J. and Vermeglio, A., eds.) NATO ASI Series, Vol. 149, 419–421Google Scholar