The Binding and Interaction of Pigments and Quinones in Bacterial Reaction Centers Studied by Infrared Spectroscopy

  • Werner Mäntele
  • Monika Leonhard
  • Michael Bauscher
  • Eliane Nabedryk
  • Gerard Berger
  • Jacques Breton
Part of the FEMS Symposium book series (FEMSS)

Abstract

The primary photochemistry in bacterial photosynthesis involves a charge separation, stable for milliseconds to seconds, between specialized pigments acting as the primary electron donor and the intermediary electron acceptor, and quinones acting as primary and secondary electron acceptors. In bacterial reaction centers, the primary electron donor (P) is a bacteriochlorophyll (BChl) a or b dimer, the intermediary acceptor (H) is a bacteriopheophytin (BPheo) a or b monomer, and ubiquinones or menaquinones have been identified as electron acceptors (Q). The specifity, efficiency and stability of this charge separation relies on the arrangement and specific interaction of the pigments and redox components in the protein matrix. X-ray structures available for bacterial RC [1,2] provide a static picture of the quiescent state and suggest specific interactions of the pigments and quinones with their host site. However, additional information on the mechanisms and dynamics of primary electron transfer and on the concomitant change of interactions and protein conformation is required. Time-resolved optical spectroscopy (for a review, see [3]) as well as resonance Raman spectroscopy (for a review, see [4]) have provided such information.

Keywords

Difference Spectrum Primary Electron Donor Primary Charge Separation Bacterial Reaction Center Difference Spectroscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    H. Michel, O. Epp and J. Deisenhofer; Pigment-Protein Interactions in the Photosynthetic Reaction Centre from Rhodopseudomonas Viridis; The EMBO Journal 5: 2445 (1986)PubMedGoogle Scholar
  2. [2]
    J.P. Allen, G. Feher, T.O. Yeates and D.C. Rees; Structure of the Reaction Center from Rhodobacter Sphaeroides R-26: The Cofactors; Proc. Natl. Acad. Sci. USA 84: 5730 (1988)CrossRefGoogle Scholar
  3. W.W. Parson; Photosynthetic Bacterial Reaction Centers; Ann. Rev. Biophys. Bioeng. 11:57 (1982)CrossRefGoogle Scholar
  4. [4]
    M. Lutz; Resonance Raman Studies in Photosynthesis; In: Advances in Infrared and Raman Spectroscopy (Clark, R.H.J and , R.E., eds.) Wiley Heyden 11:211 (1984)Google Scholar
  5. [5]
    W. Mäntele, E. Nabedryk, B.A. Tavitian, W. Kreutz, and J. Breton; Light Induced Fourier Transform Infrared (FTIR) Spectroscopic Studies of the Primary Donor Oxidation in Bacterial Photosynthesis; FEBS Letters 187: 227 (1985)CrossRefGoogle Scholar
  6. [6]
    E. Nabedryk, W. Mäntele, B.A. Tavitian and J. Breton; Light-Induced Fourier-Transform Infrared (FTIR) Spectroscopic Investigations of the Intermediary Acceptor Reduction in Bacterial Photosynthesis; Photochem. Photobiol. 43: 461 (1986)CrossRefGoogle Scholar
  7. [7]
    W. Mäntele, A.M. Wollenweber, E. Nabedryk, J. Breton; Infrared Spectroelectrochemistry of Bacteriochlorophylls and Bacteriopheophytins: Implications for the Binding of the Pigments in the Reaction Center from Photosynthetic Bacteria; Proc. Natl. Acad. Sci. USA 85: 8468 (1988)PubMedCrossRefGoogle Scholar
  8. [8]
    W. Mäntele, A.M. Wollenweber, F. Rashwan, J. Heinze, E. Nabedryk, G. Berger, J. Breton; Fourier-Transform Infrared Spectroelectrochemistry of the Bacteriochlorophyll Anion Radical; Photochem. Photobiol. 47: 451 (1988)CrossRefGoogle Scholar
  9. [9]
    K. Ballschmiter, J.J. Katz; An Infrared Study of Chlorophyll-Chlorophyll and Chlorophyll-Water Interactions; J. Am. Chem. Soc. 91: 2661 (1969)CrossRefGoogle Scholar
  10. [10]
    B.R. Clark and D.H. Evans; Infrared Studies of Quinone Radical Anions and Dianions Generated by Flow-Cell Electrolysis. J. Electroanal. Chem. 69: 181 (1976)CrossRefGoogle Scholar
  11. [11]
    W. Mäntele, F. Siebert and W. Kreutz; Kinetic Properties of Rhodopsin and Bacteriorhodopsin Measured by Kinetic Infrared Spectroscopy (KIS) Methods in Enzymology 88: 729 (1982)Google Scholar
  12. [12]
    R. Hienerwadel, W. Kreutz and W. Mäntele; Time-Resolved Infrared Spectroscopy using Tunable Diode Lasers: Characterization of Intermediates in Light-Induced Electron Transfer of Photosynthesis; Procedings of the III European Conference on the Spectroscopy of Biological Molecules, Rimini (1989), in the pressGoogle Scholar
  13. [13]
    M.S. Braiman and K.J. Rothschild; Fourier-Transform Infrared Techniques for Probing Membrane Protein Structure; Ann. Rev. Biophys. Biophys. Chem. 17: 541 (1988)CrossRefGoogle Scholar
  14. [14]
    R. Hienerwadel, W. Kreutz and W. Mäntele; A Period-Four Infrared Signal from Active Water-Splitting Complex; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm 1989, in the pressGoogle Scholar
  15. [15]
    G. Berger, A.M. Wollenweber, J. Kleo, S. Andrianambinintsoa, W. Mäntele; A Rapid Preparative Method for Purification of Bacteriochlorophyll A and B; J. Liq. Chrom. 10: 1519 (1987)CrossRefGoogle Scholar
  16. [16]
    M. Leonhard, A.M. Wollenweber, G. Berger, J. Kleo, E. Nabedryk, J. Breton and W. Mäntele; Infrared spectroscopy and Electrochemistry of Chlorophylls: Model Compound Studies on the Interaction in their Native Environment; In: Techniques and New Developments in Photosynthesis; (Barber,J. ed.) in the pressGoogle Scholar
  17. [17]
    J. Heinze; Cyclic Voltammetry — “Electrochemical Spectroscopy”; Angew. Chemie (Int. Ed. Engl.) 23:831 (1984)Google Scholar
  18. [18]
    M. Bauscher, K. Bagley, E. Nabedryk, J. Breton, W. Mäntele; Models for Ubiquinones and their Anions Involved in Photosynthetic Electron Transfer, Characterized by Thin-Layer Electrochemistry and FTIR/UV/VIS Spectroscopy; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm (1989), in the pressGoogle Scholar
  19. [19]
    R.C. Prince, D.M. Tiede, J. P. Thornber and P.L. Dutton; Spectroscopic Properties of the Intermediary Electron Carrier in the Reaction Center of Rhodopseudomonas viridis; Biochim. Biophys. Acta 462: 467 (1977)PubMedCrossRefGoogle Scholar
  20. [20]
    E. Nabedryk, S. Andrianambinintsoa, W. Mäntele, J. Breton; FTIR Investigations of the Intermediary Electron Acceptor Photoreduction in Purple Photosynthetic bacteria and Green Plants; In: The Photosynthetic Bacterial Reaction Center — Structure and Dynamics ( J. Breton & A. Vermeglio, eds) NATO ASI Series (1988), Plenum PressGoogle Scholar
  21. [21]
    M. Leonhard, E. Nabedryk, G. Berger, J. Breton, W. Mäntele; Model Compound Studies of Pigments Involved in Photosynthetic Energy Conversion: Infrared (IR) -Spectro-Electrochemistry of Chlorophylls and Pheophytins; Proceedings of the VIIIth International Congress on Photosynthesis,Stockholm (1989), in the pressGoogle Scholar
  22. [22]
    B.A. Tavitian, E. Nabedryk, W. Mäntele, J. Breton; Light-Induced FourierTransfrom Infrared (FTIR) Spectroscopic investigations of Primary Reactions in Photosystem I and Photosystem II; FEES Lett 201: 151 (1986)CrossRefGoogle Scholar
  23. [23]
    E. Nabedryk, S. Andrianambinintsoa, G. Berger, M. Leonhard, W. Mäntele, J. Breton; Characterization of Bonding Interactions of the Intermediary Electron Acceptor in the Reaction Center of Photosystem II by FTIR Spectroscopy; Manuscript submitted to BBA (July 1989)Google Scholar
  24. [24]
    K.A. Bagley, E. Abresch, M. Okamura, G. Feher, M. Bauscher, W. Mäntele, E. Nabedryk, J. Breton; FTIR studies of the D+QA and D+QB States in Reaction Centers from Rb. sphaeroides; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm (1989), in the pressGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Werner Mäntele
    • 1
  • Monika Leonhard
    • 1
  • Michael Bauscher
    • 1
  • Eliane Nabedryk
    • 2
  • Gerard Berger
    • 2
  • Jacques Breton
    • 2
  1. 1.Institut für Biophysik und StrahlenbiologieUniversität FreiburgFreiburgGermany
  2. 2.Service de Biophysique, Département de BiologieCEN SaclayGif-sur-YvetteFrance

Personalised recommendations