The Binding and Interaction of Pigments and Quinones in Bacterial Reaction Centers Studied by Infrared Spectroscopy
The primary photochemistry in bacterial photosynthesis involves a charge separation, stable for milliseconds to seconds, between specialized pigments acting as the primary electron donor and the intermediary electron acceptor, and quinones acting as primary and secondary electron acceptors. In bacterial reaction centers, the primary electron donor (P) is a bacteriochlorophyll (BChl) a or b dimer, the intermediary acceptor (H) is a bacteriopheophytin (BPheo) a or b monomer, and ubiquinones or menaquinones have been identified as electron acceptors (Q). The specifity, efficiency and stability of this charge separation relies on the arrangement and specific interaction of the pigments and redox components in the protein matrix. X-ray structures available for bacterial RC [1,2] provide a static picture of the quiescent state and suggest specific interactions of the pigments and quinones with their host site. However, additional information on the mechanisms and dynamics of primary electron transfer and on the concomitant change of interactions and protein conformation is required. Time-resolved optical spectroscopy (for a review, see ) as well as resonance Raman spectroscopy (for a review, see ) have provided such information.
KeywordsDifference Spectrum Primary Electron Donor Primary Charge Separation Bacterial Reaction Center Difference Spectroscopy
Unable to display preview. Download preview PDF.
- M. Lutz; Resonance Raman Studies in Photosynthesis; In: Advances in Infrared and Raman Spectroscopy (Clark, R.H.J and , R.E., eds.) Wiley Heyden 11:211 (1984)Google Scholar
- W. Mäntele, A.M. Wollenweber, E. Nabedryk, J. Breton; Infrared Spectroelectrochemistry of Bacteriochlorophylls and Bacteriopheophytins: Implications for the Binding of the Pigments in the Reaction Center from Photosynthetic Bacteria; Proc. Natl. Acad. Sci. USA 85: 8468 (1988)PubMedCrossRefGoogle Scholar
- W. Mäntele, F. Siebert and W. Kreutz; Kinetic Properties of Rhodopsin and Bacteriorhodopsin Measured by Kinetic Infrared Spectroscopy (KIS) Methods in Enzymology 88: 729 (1982)Google Scholar
- R. Hienerwadel, W. Kreutz and W. Mäntele; Time-Resolved Infrared Spectroscopy using Tunable Diode Lasers: Characterization of Intermediates in Light-Induced Electron Transfer of Photosynthesis; Procedings of the III European Conference on the Spectroscopy of Biological Molecules, Rimini (1989), in the pressGoogle Scholar
- R. Hienerwadel, W. Kreutz and W. Mäntele; A Period-Four Infrared Signal from Active Water-Splitting Complex; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm 1989, in the pressGoogle Scholar
- M. Leonhard, A.M. Wollenweber, G. Berger, J. Kleo, E. Nabedryk, J. Breton and W. Mäntele; Infrared spectroscopy and Electrochemistry of Chlorophylls: Model Compound Studies on the Interaction in their Native Environment; In: Techniques and New Developments in Photosynthesis; (Barber,J. ed.) in the pressGoogle Scholar
- J. Heinze; Cyclic Voltammetry — “Electrochemical Spectroscopy”; Angew. Chemie (Int. Ed. Engl.) 23:831 (1984)Google Scholar
- M. Bauscher, K. Bagley, E. Nabedryk, J. Breton, W. Mäntele; Models for Ubiquinones and their Anions Involved in Photosynthetic Electron Transfer, Characterized by Thin-Layer Electrochemistry and FTIR/UV/VIS Spectroscopy; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm (1989), in the pressGoogle Scholar
- E. Nabedryk, S. Andrianambinintsoa, W. Mäntele, J. Breton; FTIR Investigations of the Intermediary Electron Acceptor Photoreduction in Purple Photosynthetic bacteria and Green Plants; In: The Photosynthetic Bacterial Reaction Center — Structure and Dynamics ( J. Breton & A. Vermeglio, eds) NATO ASI Series (1988), Plenum PressGoogle Scholar
- M. Leonhard, E. Nabedryk, G. Berger, J. Breton, W. Mäntele; Model Compound Studies of Pigments Involved in Photosynthetic Energy Conversion: Infrared (IR) -Spectro-Electrochemistry of Chlorophylls and Pheophytins; Proceedings of the VIIIth International Congress on Photosynthesis,Stockholm (1989), in the pressGoogle Scholar
- E. Nabedryk, S. Andrianambinintsoa, G. Berger, M. Leonhard, W. Mäntele, J. Breton; Characterization of Bonding Interactions of the Intermediary Electron Acceptor in the Reaction Center of Photosystem II by FTIR Spectroscopy; Manuscript submitted to BBA (July 1989)Google Scholar
- K.A. Bagley, E. Abresch, M. Okamura, G. Feher, M. Bauscher, W. Mäntele, E. Nabedryk, J. Breton; FTIR studies of the D+QA− and D+QB− States in Reaction Centers from Rb. sphaeroides; Proceedings of the VIIIth International Congress on Photosynthesis; Stockholm (1989), in the pressGoogle Scholar