Limited Proteolysis and Its Influence on Thermal Stability of the Photosynthetic Reaction Center from Chloroflexus aurantiacus
Limited proteolysis with proteases of different specificity has been used to obtain a number of preparations of the reaction center (RC) from the thermophilic green bacterium Chloroflexus aurantiacus. The absorption spectrum of the native RC are preserved in these preparations, even after the shortening of the N-termini of both RC subunits to different extent. Considerable part of the hydrophilic N-terminus of the L-subunit has been shown to be unaccessible to the proteases. AlaL3-ArgL25 region contribute to the thermal stability of the Chloroflexus RC.
KeywordsPurple Bacterium Limited Proteolysis Phototrophic Bacterium Photosynthetic Reaction Center Primary Charge Separation
Unable to display preview. Download preview PDF.
- 1.C. R. Woese, Bacterial evolution, Microbiol. rev. 5I: 22 I (1987)Google Scholar
- 5.J. A. Shiozawa, F. Lottspeich, D. Oesterhelt, and R. Feick, The primary structure of the Chioroflexus aurantiacus reaction-center polypeptides, Eur. J. Biochem. Ï80: 75 (1989)Google Scholar
- 6.M. D. Mamedov, N. I. Zacharova, A. A. Kondrashin, and A. Yu. Semenov, The effect of o-phenanthroline on electrogenesis under primary charge separation in the reaction center of Rhodopseudomonas sphaeroides, Biol. Membrany (Russ) 3: 513 (1986)Google Scholar