Carotenoid Absorbance Changes in Liposomes Reconstituted with Pigment-Protein Complexes from Rhodobacter Sphaeroides
The electrochromic behaviour of carotenoids has been widely used to determine the electrical potential difference (∆ψ) across photosynthetic membranes, like chromatophores  and bacterial cells [2,3]. The carotenoid absorbance change has several advantages over other methods for recording the ∆ψ: (i) the method is non-invasive, (ii) the relationship between the ∆ψ and the absorbance change is linear and (iii) the method shows a rapid response time. The major disadvantage of the carotenoid band shift as a ∆ψ indicator is that a calibration of the ∆ψ dependent bandshifts is not (always) possible in every experimental system .
KeywordsDifference Spectrum Absorbance Change Photosynthetic Membrane Electrical Potential Difference Rhodopseudomonas Palustris
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