Skip to main content

Preliminary Studies on the Operon Coding for the Reaction Center Polypeptides in Chloroflexus Aurantiacus

  • Chapter
Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria

Part of the book series: FEMS Symposium ((FEMSS))

Abstract

The isolated reaction center of the thermophilic, facultative photoheterotroph Chloroflexus (C.) aurantiacus is composed of two polypeptides (Shiozawa et al., 1987). The genes encoding the two polypeptides have been cloned and sequenced (Shiozawa et al., 1989 and Ovchinnikov et al., 1988 a, b). The two genes are adjacent to one another; 17 bases separate the stop codon of the L-gene and the start codon of the M-gene. The deduced amino acid sequences are about 40% similar to the respective subunits of Rhodobacter sphaeroides. We have now undertaken studies to determine if the puf operon of this green nonsulfur bacteria is similar to that of Rhodospirillaceae.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adams, C. W., Forrest, M. E., Cohen, S. N., and Beatty, J. T., 1989, Structural and functional analysis of transcriptional control of the Rhodobacter capsulatus puf operon. J. Bacteriol. 171, 473.

    PubMed  CAS  Google Scholar 

  • Aiba, H., Adhya, S., and de Crombrugghe, B., 1981, Evidence for two functional gal promoters in intact Escherichia col cells, J.

    Google Scholar 

  • Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Smith, Seidman, J. G,. and Struhl, K., 1988, “Current Protocols in Molecular Biology 1987–1988,” John Wiley and Sons, New York.

    Google Scholar 

  • Bauer, C. E., Young, D. A., anad Marrs, B. L., 1988, Analysis of the Rhodobacter capsula tus puf operon. Location of the oxygen-regulated promoter region and the identification of an additional puf-encoded gene, J. Biol. Chem. 263, 4820.

    PubMed  CAS  Google Scholar 

  • Castenholz, R. W. and Pierson, B. K., 1981, Isolation of members of the family Chloroflexaceae, in: “The Prokaryotes. A Handbook of Habitats, Isolation, and Identification of Bacteria,” M. P. Starr, H. Stolp, H. G., Trüper, A. Balows, and H. G. Schlegel, eds., Springer-Verlag, Berlin.

    Google Scholar 

  • Feick, R. G. and Fuller, R. C., 1984, Topography of the photosynthetic apparatus of Chloroflexus aurantiacus, Biochemistry 23: 3693.

    Article  CAS  Google Scholar 

  • Hennighausen, L and Lubon, H., 1987, Interaction of protein witn DNA in vitro, in: “Methods in Enzymology,” S. L. Berger and A. R. Kimmel, eds., Academic Press, Orlando Vol. 152.

    Google Scholar 

  • Ovchinnikov, Yu. A., Abdulaev, N. G., Zolotarev, A. S., Shmukler, B. E., Zargarov, A. A., Kutuzov, M. A., Telezhinskaya, I. N., and Levina, N. B., 1988, Photosynthetic reaction centre of Chloroflexus aurantiacus. I. Primary structure of L-subunit, FEBS Lett., 231: 237.

    Article  PubMed  CAS  Google Scholar 

  • Ovchinnikov, Yu. A., Abdulaev, N. G., Shmukler, B. E., Zargarov, A. A., Kutuzov, M. A., Telezhinskaya, I.N., Levina, N. B., and Zolotarev, A. S., 1988, Photosynthetic reaction centre of Chloroflexus aurantiacus. Primary structure of M-subunit, FEBS Lett., 232: 364.

    Article  PubMed  CAS  Google Scholar 

  • Shiozawa, J. A., Lottspeich, F., and Feick, R., 1987, The photochemical reaction center of Chloroflexus aurantiacus is composed of two structurally similar polypeptides, Eur. J. Biochem. 167: 595.

    Article  PubMed  CAS  Google Scholar 

  • Shiozawa, J. A., Lottspeich,.F., Oesterhelt, D. and Feick, R., 1989, The primary structure of the Chloroflexus auran tiacus reaction center polypeptides, Eur. J.Biochem. 180: 75.

    Article  PubMed  CAS  Google Scholar 

  • Tavers, A. A., 1987, Structure and function of E. coli promoter DNA, CRC Critical Reviews….n Biochemistry 22, 181.

    Article  Google Scholar 

  • Wechsler, T., Brunisholz, R., Suter, F., Fuller, R. C., and Zuber, H., 1985, The complete amino acid sequence of a bacteriochlorophyll a binding polypeptide isolated from the cytoplasmic membranes of the green photosynthetic bacterium Chloroflexus aurantiacus, FEBS Lett. 191: 34.

    Article  CAS  Google Scholar 

  • Wechsler, T. D., Brunisholz, R. A. Frank, G., Suter, F., and Zuber, H., 1987, The complete amino acid sequence of the antenna polypeptide B806–866–0 from the cytoplasmic membrane of the green bacterium Chloroflexus aurantiacus, FEBS Lett. 210: 189.

    Article  CAS  Google Scholar 

  • Youvan, D. C., Byline, E. J., Alberti, M., Begusch, H., and Hearst, J. E., 1984, Nucleotide and deduced polypeptide sequences of the photosynthetic reaction center, B870 antenna, and flanking polypeptides from R. capsulate, Cell 37, 949.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer Science+Business Media New York

About this chapter

Cite this chapter

Shiozawa, J.A., Csiszȧr, K., Feick, R. (1990). Preliminary Studies on the Operon Coding for the Reaction Center Polypeptides in Chloroflexus Aurantiacus . In: Drews, G., Dawes, E.A. (eds) Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria. FEMS Symposium. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0893-6_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-0893-6_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0895-0

  • Online ISBN: 978-1-4757-0893-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics