Abstract
Among the photosynthetic bacteria, Rhodobacter capsulatus has been particularly well characterized genetically (reviewed in Scolnik and Marrs, 1987). By a variety of fine structure genetic mapping techniques, it has been previously determined that most of the genes involved in the synthesis of the photosynthetic apparatus in this organism are clustered in a small region of the genome (Yen and Marrs, 1976), and that region has been cloned (Marrs, 1981; Taylor, et al., 1983)) and some of the genes sequenced (Youvan, et al., 1984). Mapping by R plasmid-mediated conjugation has produced a circular linkage map of the chromosome (Willison, et al., 1985). A detailed study of the overall organization of the chromosome of R. capsulatus should assist in the understanding of the global regulation of its genes, and the availability in cloned form of all parts of the genome will facilitate the identification and sequencing of additional genes. Towards these ends we are determining a physical map of the chromosome of R. capsulatus.
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References
Avtges, P., Scolnik, P. A., and Haselkorn, R., 1983, Genetic and physical map of the structural genes (nifH,D,K) coding for the nitrogenase complex of Rhodopseudomonas capsulata, J. Bacteriol., 156: 251.
Coulson, A., Sulston, J., Brenner, S., and Kam, J., 1986, Toward a physical map of the genome of the nematode Caenorhabditis elegans, Proc. Natl, Acad. Sci. USA, 83: 7821.
Daldal, F., Cheng, S., Applebaum, J., Davidson, E. and Prince, R. C., 1986, Cytochrome c2 is not essential for photosynthetic growth of Rhodopseudomonas capsulata, Proc. Natl. Acad. Sci. USA, 83: 2012.
Daldal, F., Davidson, E., and Cheng, S., 1987, Isolation of the structural genes for the Rieske Fe-S protein, cytochrome b and cytochrome c 1, all components of the ubiquinol:cytochrome c2 oxidoreductase complex of Rhodopseudomonas capsulata, J. Mol. Biol., 195: 1.
Feinberg, A. P. and Vogelstein, B., 1983, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Anal. Biochem., 132: 6.
Gibson, T. J., Coulson, A. R., Sulston, J. E., and Little, P. F. R., 1987, Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect of DNA yield and cloned insert frequency, Gene, 53: 275.
Jackson, D. A. and Cook, P. R., 1985, A general method for preparing chromatin containing intact DNA, EMBO J„ 4: 913.
Kam, J., Matthes, H. W. D., Gait, M. J. and Brenner, S., 1984, A new selective phage cloning vector, X2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI, Gene, 32: 217.
Maniatis, T., Fritsch, E. F., and Sambrook, J., 1982, “Molecular Cloning: A Laboratory Manual,” Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Marrs, B. L., 1981, Mobilization of the genes for photosynthesis from Rhodopseudomonas capsulata by a promiscuous plasmid, J. Bacteriol., 146: 1003.
McClelland, M., Jones, R., Patel, Y., and Nelson, M., 1987, Restriction endonucleases for pulsed field mapping of bacterial genomes Nucleic Acids Res., 15: 5985.
Purrello, M. and Balazs, I., 1983, Direct hybridization of labeled DNA to DNA in agarose gels, Anal. Biochem., 128: 393.
Schumann, J. P., Waitches, G. M., and Scolnik, P. A., 1986, A DNA fragment hybridizing to a nif probe in Rhodobacter capsulatus is homologous to a 16S rRNA gene, Gene, 48: 81.
Scolnik, P. A. and Marrs, B. L., 1987, Genetic research with photosynthetic bacteria, Ann. Rev. Microbiol., 41: 703.
Scolnik, P. A., Virosco, J., and Haselkorn, R., 1983, The wild-type gene for glutamine synthetase restores ammonia control of nitrogen fixation to Gln’ (glnA) mutants of Rhodopseudomonas capsulata, J. Bacteriol., 155: 180.
Sulston, J., Mallett, F., Staden, R., Durbin, R., Horsnell, T., and Coulson, A., 1988, Software for genome mapping by fingerprinting techniques, CABIOS, 4: 125.
Taylor, D. P., Cohen, S. N., Clark, W. G., and Mans, B. L., 1983, Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique, J. Bacteriol. 154: 580.
Tybulewicz, V. L. J., Falk, G., and Walker, J. E., 1984, Rhodopseudomonas blastica atp operon, nucleotide sequence and transcription, J. Mol, Biol., 179: 185.
Willison, J. C., Ahombo, G., Chabert, J., Magnin, J.-P., and Vignais, P. M., 1985, Genetic mapping of the Rhodopseudomonas capsulata chromosome shows non-clustering of genes involved in nitrogen fixation, J. Gen. Micro., 131: 3001.
Yen, H-C., and Mans, B. L., 1976, Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsula ta, J. Bacteriol„ 126: 619.
Youvan, D. C., Bylina, E. J., Alberti, M., Begusch, H., and Hearst, J. E., 1984, Nucleotide and deduced polypeptide sequences of the photosynthetic reaction center, B870 antenna, and flanking polypeptides from R. capsulata, Cell, 37: 949.
Youvan, D. C. and Ismail, S., 1985, Light-harvesting II (B800–850 complex) structural genes from Rhodopseudomonas capsulata, Proc. Natl. Acad. Sci. USA 82: 58.
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Williams, J.C., Marrs, B.L., Brenner, S. (1990). Physical Mapping of the Genome of Rhodobacter Capsulatus . In: Drews, G., Dawes, E.A. (eds) Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria. FEMS Symposium. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0893-6_2
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DOI: https://doi.org/10.1007/978-1-4757-0893-6_2
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