Abstract
The events which surround fertilization and ultimately result in the beginning of the developmental program are referred to as “egg activation”, as attested by the title of this chapter. However, the definition of activation is “to make active” and the first item I discuss is whether this term is appropriate for what occurs at fertilization. I do not ask this to be pedantic or pugnacious, but because a search for a more appropriate term might lead to a better definition/description and understanding of fertilization.
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References
Allemand, D., G. De Renzis, P. Payan, and J-P. Girard. 1986. Regulatory and energetic role of Na` in amino acid uptake by fertilized sea urchin eggs. Dev. Biol. 118: 19–27.
Allemand, D., B. Ciapa, and G. De Renzis. 1987a. Effect of cytochalasin B on the development of membrane transports in sea urchin eggs after fertilization. Dev. Growth & Differ. 29: 333–340.
Allemand, D., G. De Renzis, J-P. Girard, and P. Payan. 1987b. Activation of amino acid uptake at fertilization in the sea urchin egg. Requirement for proton compartmentalization during cytosolic alkalosis. Exp. Cell Res. 169: 169–177.
Begg, D., L. J. Rebhun, and H. Hyatt. 1982. Structural organization of actin in the sea urchin egg cortex: microvillar elongation in the absence of actin bundle formation. J. Cell Biol. 93: 24–32.
Brooks, S. C. and E. L. Chambers. 1948. Penetration of radioactive phosphate into the eggs of
Strongylocentrotus purpuratus, S. franciscanus and Urechis caupo. Biol. Bull. 95:262–263. Brooks, S. C. and E. L. Chambers. 1954. The penetration of radioactive phosphate into marine eggs. Biol. Bull. 106:279–296.
Byrd, W. and G. Perry. 1980. Cytochalasin B blocks sperm incorporation but allows activation of the sea urchin egg. Exp. Cell Res. 126: 333–342.
Carron, C. P. and F. J. Longo. 1982. Relation of cytoplasmic alkalinization to microvillar
elongation and microfilament formation in the sea urchin egg. Dev. Biol. 89:128–137. Chambers, E. L. 1975. Na` is required for nuclear and cytoplasmic activation of sea urchin eggs by sperm and divalent ionophores. J. Cell Biol. 67:60a.
Chambers, E. L. 1976, Na’ is essential for activation of the inseminated sea urchin egg. J. Exp. Zool. 197: 149–154.
Chambers, E. L. and R. Chambers. 1949. Ion exchanges and fertilization in echinoderm eggs. Am. Nat. 83: 269–284.
Chambers, E. L. and R. E. Henkley. 1979. Non-propagated cortical reaction induced by the divalent ionophore A23187 in eggs of the sea urchin, Lytechinus variegatus. Exp. Cell Res. 124: 441–446.
Chambers, E. L., B. C. Pressman, and B. Rose. 1974. The activation of sea urchin eggs by divalent ionophores A23187 and X-537A. Biochem. Biophys. Res. Commun. 60: 126–132.
Chambers, E. L. and W. E. White. 1949. The accumulation of phosphate and evidence for synthesis of adenosine triphosphate in the fertilized sea urchin egg. Biol. Bull. 97: 225–226.
Chambers, E. L. and W. E. White. 1954. The accumulation of phosphate by fertilized sea urchin eggs. Biol. Bull. 106: 297–307.
Chambers, E. L. and A. H. Whiteley. 1966. Phosphate transport in fertilized sea urchin eggs. I. Kinetic aspects. J. Cell. Physiol. 68: 289–308.
Ciapa, B. and M. Whitaker. 1986. Two phases of inositol polyphosphate and diacylglycerol production at fertilization. FEBS Lett. 195: 247–351.
Ciapa, B., D. Allemand, P. Payan, and J. P. Girard. 1984. Sodium-potassium exchange in sea urchin egg. I. Kinetic and biochemical characterization at fertilization. J. Cell. Physiol. 121: 235–242.
Dayson, H. 1959. A Textbook of General Physiology. Little, Brown and Co., Boston.
Dubé, F. and D. Epel. 1986. The relation between intracellular pH and rate of protein synthesis in sea urchin eggs and the existence of a pH-independent event triggered by ammonia. Exp. Cell Res. 162: 191–204.
Dubé, F., T. Schmidt, C. H. Johnson, and D. Epel. 1985. The hierarchy of requirements for an elevated intracellular pH during early development of sea urchin embryos. Cell 40: 657–666.
Dunphy, W. G. and J. W. Newport. 1988. Unraveling of mitotic control mechanisms. Cell 55: 925–928
Epel, D. 1972. Activation of an Nat-dependent amino acid transport system upon fertilization of sea urchin eggs. Exp. Cell Res. 72: 74–89.
Epel, D. 1980. Experimental analysis of the role of intracellular calcium in the activation of the sea urchin egg at fertilization p. 169–186. In: The Cell Surface: Mediator of Developmental Processes. ( S. Subtelny and N. K. Wessells (Eds.). Academic Press, New York.
Epel, D. 1988. The role of Na`-H` exchange and intracellular pH changes in fertilization. In: Na`-H’ Exchange. S. Grinstein (Ed.) CRC Press, Boca Raton. ( In press ).
Epel, D. and F. Dubé. 1987. Intracellular pH and cell proliferation p. 364–394. In: Control of Animal Cell Proliferation. A. Boynton and H. L. Leffert (Eds.). Academic Press, Orlando.
Epel, D. and J. D. Johnson. 1976. Reorganization of the sea urchin egg surface at fertilization and its relevance to the activation of development. p. 105–120. In: Biogenesis and Turnover of Membrane Molecules. J. S. Cook (Ed.) Raven Press, New York.
Epel, D., C. Patton, R. W. Wallace, and W. Y. Cheung. 1981. Calmodulin activates NAD kinase of sea urchin eggs; an early event of fertilization. Cell 23: 543–549.
Epel, D., R. Steinhardt, T. Humphreys, and D. Mazia. 1974. An analysis of the partial metabolic derepression of sea urchin eggs by ammonia. The existence of independent pathways. Dev. Biol. 40: 245–255.
Grainger, J. L., M. M. Winkler, S. S. Shen, and R. A. Steinhardt. 1979. Intracellular pH controls protein synthesis rate in sea urchin egg and early embryo. Dev. Biol. 68: 396–406.
Hamaguchi, Y. and Y. Hiramoto. 1981. Activation of sea urchin eggs by microinjection of calcium buffers. Exp. Cell Res. 134: 171–179.
Ishihara, K. 1968. An analysis of acid polysaccharides produced at fertilization of sea urchin eggs. Exp. Cell Res. 51: 473–484.
Isono, N. 1963. Carbohydrate metabolism in sea urchin eggs IV. Intracellular localization of enzymes of the pentose phosphate cycle in unfertilized and fertilized eggs. J. Fac. Sci. Univ. Tokyo 10: 37–53.
Isono, N. and I. Yasumasu. 1968. Pathways of carbohydrate breakdown in sea urchin eggs. Exp. Cell Res. 50: 616–626.
Johnson, C. H. and D. Epel. 1981. Intracellular pH of sea urchin eggs measured by the DMO method. J. Cell Biol. 89: 284–291.
Johnson, J. D., D. Epel, and M. Paul. 1976. Na`-H’ exchange is required for activation of sea urchin eggs after fertilization. Nature (Load.) 262: 661–664.
Lee, H. C. and D. Epel. 1983. Changes in intracellular acidic compartments in sea urchin eggs after activation. Dev. Biol. 98: 446–454.
Longo, F. J. 1978. Effects of cytochalasin B on sperm-egg interactions. Dev. Biol. 67:249–265. Mar, H. 1980. Radial cortical fibers and pronuclear migration in fertilized and artificially activated eggs in Lytechinus pictus. Dev. Biol. 78: 1–13.
Nakazawa, T., K. Asami, R. Shoger, A. Fujiwara, and I. Yasumasu. 1970. Ca+2 uptake, H+ ejection and respiration in sea urchin eggs on fertilization. Exp. Cell Res. 65: 143–146.
Nishioka, D. and N. Cross. 1978. The role of external sodium in sea urchin fertilization. p. 403–414. In: Cell Reproduction. E. R. Dirksen, D. M. Prescott, and C. F. Fox (Eds.) Academic Press, New York.
Paul, M. and D. Epel. 1975. Formation of fertilization acid by sea urchin eggs does not require specific cations. Exp. Cell Res. 94: 1–6.
Paul, M., J. D. Johnson, and D. Epel. 1976. Fertilization acid of sea urchin eggs is not a consequence of cortical granule exocytosis. J. Exp. Zool. 197: 127–133.
Piatigorsky, J. and A. H. Whiteley. 1965. A change in permeability and uptake of C14-uridine in response to fertilization in Strongylocentrotus purpuratus eggs. Biochim. Biophys. Acta 108: 404–418.
Poenie, M., J. Alderton, R. Tsien, and R. Steinhardt. 1985. Changes of free calcium levels with stages of the cell division cycle. Nature (Lond.) 315: 147–149.
Schatten, G., T. Bestor, R. Balczon, J. Henson, and H. Schatten. 1985. Intracellular pH shift leads to microtubule assembly and microtubule-mediated motility during sea urchin fertilization: correlations between elevated intracellular pH and microtubule disassembly. Eur. J. Cell Biol. 36: 116–127.
Schneider, E. G. 1985. Activation of Nay-dependent transport at fertilization in the sea urchin: requirements of both an early event associated with exocytosis and a later event involving increased energy metabolism. Dev. Biol. 108: 152–163.
Shen, S. S. and R. A. Steinhardt. 1978. Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg. Nature (Load.) 272: 253–254.
Shen, S. S. and R. A. Steinhardt. 1979. Intracellular pH and the sodium requirement at fertilization. Nature (Loud.) 282: 87–89.
Spudich, A., J. T. Wrenn, and N. K. Wessells. 1988. Unfertilized sea urchin eggs contain a discrete cortical shell of actin that is subdivided into two organizational states. Cell Motil. Cytoskeleton 9: 85–96.
Steinhardt, R. A. and D. Epel. 1974. Activation of sea urchin eggs by a calcium ionophore. Proc. Natl. Acad. Sci. USA 71: 1915–1919.
Steinhardt, R. A. and D. Mazia. 1973. Development of K+-conductance and membrane potentials in unfertilized sea urchin eggs after exposure to NH2OH. Nature (Lond.) 241: 400–401.
Steinhardt, R. A., R. Zucker, and G. Schatten. 1977. Intracellular calcium release at fertilization in the sea urchin egg. Der. Biol. 58: 185–196.
Suprynowicz, F. A. and D. Mazia. 1985. Fluctuation of the Ca+2-sequestering activity of permeabilized sea urchin embryos during the cell cycle. Proc. Natl. Acad. Sci. USA 82: 2389–2393.
Swann, K. and M. J. Whitaker. 1986. The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs. J. Cell Biol. 103: 2333–2342.
Swann, K., B. Ciapa, and M. Whitaker. 1987. Cellular messengers and sea urchin egg activation. p. 45–69. In: Molecular Biology of Invertebrate Development. J. D. O’Connor (Ed.). Alan R. Liss, New York.
Swezey, R. R. and D. Epel. 1986. Regulation of glucose-6-phosphate dehydrogenase activity in sea urchin eggs by reversible association with cell structural elements. J. Cell Biol. 103: 1509–1515.
Swezey, R. R. and D. Epel. 1988. Enzyme stimulation upon fertilization is revealed in electrically permeabilized sea urchin eggs. Proc. Natl. Acad. Sci. 85: 812–816.
Swezey, R. R., T. Schmidt, and D. Epel. 1987. Effects of hydrostatic pressure on actin assembly and initiation of amino acid transport upon fertilization of sea urchin eggs. p.95–111. In: Current Perspectives in High Pressure Biology. H. W. Jannasch, R. E. Marquis, and A. M. Zimmerman (Eds.). Academic Press, London.
Turner, E. L., J. Hager, and B. M. Shapiro. 1988. Ovothinol replaces glutathione per’oxidaseas a hydrogen peroxide scavenger in sea urchin eggs. Science 242: 939–941.
Vacquier, V. D. 1981. Dynamic changes of the egg cortex. Dev. Biol. 84: 1–26.
Whitaker, M. J. and R. A. Steinhardt. 1981. The relation between the increase in reducednicotinamide nucleotides and the initiation of DNA synthesis in sea urchin eggs. Cell 25: 95–103.
Whitaker, M. J. and R. A. Steinhardt. 1985. Ionic signaling in the sea urchin egg at fertilization. p. 168–222. In: Biology of Fertilization, Vol. 3. C. B. Metz and A. Monroy (Eds.). Academic Press, Orlando.
Whiteley, A. H. and E. L. Chambers. 1961. The differentiation of a phosphate transport mechanism in the fertilized egg of the sea urchin. p. 387–401. In: Symposium on Germ Cells and Development. Institut Intern. d’Embryologie and Fondazione A. Baselli.
Winkler, M. M., E. Nelson, C. Lashbrook, and J. W. B. Hershey. 1982. 31P- NMR study of the activation of the sea urchin egg. Exp. Cell Res. 139: 217–222.
Winkler, M. M., R. A. Steinhardt, J. L. Grainger, and L. Minning. 1980. Dual ionic controlsfor the activation of protein synthesis at fertilization. Nature (Loud.) 287: 558–560.
Zucker, R. S. and R. A. Steinhardt. 1978. Prevention of the cortical reaction in fertilized seaurchin eggs by injecting calcium-chelating ligands. Biochim. Biophys. Acta 541: 459–466.
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Epel, D. (1989). An Ode to Edward Chambers: Linkages of Transport, Calcium and pH to Sea Urchin Egg Arousal at Fertilization. In: Nuccitelli, R., Cherr, G.N., Clark, W.H. (eds) Mechanisms of Egg Activation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0881-3_14
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DOI: https://doi.org/10.1007/978-1-4757-0881-3_14
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