Defective RNAs of Alphaviruses
Most viruses, when passaged at high multiplicity in cultured cells, accumulate deletion mutants characterized by their ability to interfere with the replication of the standard virus. These mutants are defined as defective interfering (DI) particles (Huang and Baltimore, 1970; Perrault, 1981). One of their hallmarks is the specificity of their inhibition; they interfere only with the replication of homologous or closely related viruses. Why study DI particles? What can they tell us about the standard virus or about virus—host interactions? The following points attempt to answer these questions and provide the framework for this chapter.
KeywordsPersistent Infection Vesicular Stomatitis Virus Chicken Embryo Fibroblast Semliki Forest Virus Sindbis Virus
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