High Resolution Low Temperature Spectrophotometry of Cytochromes c

  • W. B. Elliott
  • E. Margoliash
Part of the Developments in Applied Spectroscopy book series (DAIS, volume 9)


Over the post ten years accessories for the taking of low temperature spectra in the Cary Model 11 and 14 Recording Spectrophotometers ranging from foam box Dewars to vacuum space Dewar systems have been developed. Use of an on-line computer for the summation of spectra recorded from low temperature specimens has been made to increase resolution and sensitivity of the spectrophotometers. Originally the summation operation was a manual procedure. Recently it has been completely automated for the Cary 14 by the use of the repetitive scan accessory and interface accessories developed in this laboratory. The application of these techniques to a large series of reduced crystalline cytochromes cs has led to a comparison of the separation (0–4 mμ) between the alpha 1 and alpha 2 peaks in the cytochromes from various species, to the resolution of the beta absorption peaks into as many as nine components, to the successful demonstration of the Beta prime peaks and to the consistent demonstration of a shoulder an the red side of the Soret band of all of the reduced cytochrome c’s.


Soret Band Neutral Density Filter King Penguin Wavelength Error Cary Model 
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Copyright information

© Chicago Section of the Society for Applied Spectroscopy 1971

Authors and Affiliations

  • W. B. Elliott
    • 1
  • E. Margoliash
    • 2
  1. 1.Department of BiochemistryState University of New York at BuffaloBuffaloUSA
  2. 2.Biochemical Research DepartmentAbbott LaboratoriesNorth ChicagoUSA

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