Fc Receptor-Ligand Interaction Induces Activation of Human Natural Killer Cells
Interaction of NK cells with Sepharose-linked anti-Fc receptor (FcR, CD16 antigen) antibodies inhibits their ability to mediate both spontaneous and antibody-dependent cytotoxicity, whereas bivalent anti-CD16 antibodies enhance spontaneous cytotoxicity. Treatment of cultured NK cells with Sepharose-linked anti-CD16 antibodies induces expression of IL-2 and transferrin (Tf) receptors and of 4F2 antigens and this effect is enhanced in the presence of IL-2 during treatment. Analogously, treatment of NK cells with the anti-CD16 antibodies or immune complexes synergizes with IL-2 in inducing production of IFNγ and TNF. The combined treatment of NK cells with IL-2 and anti-CD16 antibodies induces cytoplasmic accumulation of mRNA transcripts for IL-2R and for the two lymphokines. Nuclear run-on experiments show that gene transcription is induced by the two stimuli. These data indicate that FcR are specialized molecules on NK cell surface that mediate signal transduction upon ligand binding. These effects may be similar to those induced in NK cells upon target cell recognition.
KeywordsLarge Granular Lymphocyte Human Natural Killer Cell Daudi Cell Peripheral Blood Mononuclear Cell Culture Peripheral Blood Natural Killer Cell
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