Use of Congenic and Recombinant Inbred Mouse Strains to Link a Highly Conserved Idiotype Marker to an Igh-C Region Allotype Locus and to Map This New Member of the Q52 Gene Family Within the Igh-V Gene Complex
The gene coding for a highly conserved idiotype marker associated with the murine response to the hapten phthalate (Xmp) was linked to the IgCH allotype locus using a pair of congenic resistant mouse strains, CB.20 and BC.8. These two strains of mice produce the same level of anti-phthalate antibody when immunized with phthalate-KLH. However, the idiotype marker identified as CRI-Xmp was found in the anti-phthalate antibody obtained from BC.8 but not CB.20 immune mice. The presence or absence of the CRI-Xmp in the phthalate immune sera of several different wild mouse populations was also monitored. In these studies, we observed some nonconcordance in the expression of the idiotype and the heavy chain allotype marker. This nonconcordance is most likely due to a crossover event occuring between Igh-C and Igh-V gene complexes. Using BXD recombinant inbred strains, the VH-Xmp was mapped with respect to other known VH gene markers. Based upon these studies VH-Xmp was found to map close to the constant region immunoglobulin gene cluster, i.e., between VH-S14 and VH-Ox. Preliminary sequence data indicate the VH-Xmp is a member of the Q52 gene family.
KeywordsInbred Strain Immune Seron Antiidiotypic Antibody Congenic Mouse Strain Immunoglobulin Heavy Chain Variable Region
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