H-2 Antigens pp 659-670 | Cite as

Monoclonal H-2 Class I Specific Antibodies Isolated After Immunization of C57B1/6 Mice with Syngeneic Sendai Virus-Coated Cells

  • F. Kievits
  • A. Opolski
  • W. J. Boerenkamp
  • M. Pla
  • P. Ivanyi
Part of the NATO ASI Series book series (NSSA, volume 144)

Abstract

Two alloreactive H-2 (class I) specific cytotoxic IgM monoclonal antibodies (McAbs), B6-1 and B6-2, were isolated from C57B1/6 (B6) mice after injections of syngeneic Sendai virus-coated (SV+) spleen cells. McAb B6-I recognizes the public H-2 class I specificity H-2.25, shared by the H-2k(Kk) and H-2r haplotypes and cross-reacts with H-2z antigens on SV-infected (SVi) lymphoblasts. McAb B6-2 recognizes the private H-2Kf determinant H-2.26 on normal Sendai virus-negative (SV-) spleen cells but cross-reacts with H-2Kk and H-2Dd antigens on SVi blast cells. A polymorphic reaction pattern, that was dependent on the presence of Sendai virus, as tested on a panel of B10 target cells, was also observed with the cytotoxic immune sera from the mice whose cells were used in fusion experiments. Given the fact that each immune serum is an extensive mixture of anti-H-2 antibodies with different specificities, the isolated McAbs can be seen as illustrative examples which explain the reaction pattern of the immune sera. The recognition of polymorphic determinants on SV-infected, positive cells, is presumably due to a modified exposure of cross-reacting public H-2 class I determinants. Taken together, the lymphocytotoxic McAbs induced by injections of syngeneic SV+ cells recognize neither viral antigen nor self-MHC+X composite determinants, but allogeneic H-2 determinants as presented on normal, SV-coated or SV-infected cells.

Keywords

Spleen Cell Reaction Pattern Foreign Antigen Sendai Virus Fusion Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allen, H., Wraith, d., Pala, P., Askonas B., and Flavell, R.A.: Domain interactions of H-2 class I antigens after cytotoxic T-cell recognition sites. Nature 309: 279–281, 1984PubMedCrossRefGoogle Scholar
  2. Ashwell, J.D., and Schwartz, R.H.: T-cell recognition of antigen and Ia molecules as ternary complex. Nature 320: 176–179, 1986PubMedCrossRefGoogle Scholar
  3. Babbit, B.P., Allen, P.M., Matsueda, G., Haber, E., and Unanue, E.R.: Binding of immunogenic peptides to Ia histocompatibility molecules. Nature 317: 357–316, 1985.Google Scholar
  4. Cerny-Provaznik, R., van Mourik, P., Limpens, J., Leupers, T., and Ivanyi, P.: Anti-MHC immunity detected prior intentional alloimmunization. III. Natural autoreactive H-2 specific antibodies. Immunogenetics 21: 461–504, 1985CrossRefGoogle Scholar
  5. Froscher, B.G., and Klinman, N.R.: Immunization with SV40-transformed cells yields mainly MHC-restricted monoclonale antibodies. J. Exp. Med. 164: 196–210, 1986.PubMedCrossRefGoogle Scholar
  6. Gorczynski, R.M., Kennedy, M.J., MacRae, S., Steele, E.J., and Cunningham, A.J.: Restriction of antigen recognition in mouse B lymphocytes by genes mapping within the major histocompatibility complex. J. Immunol. 124: 590–596, 1980PubMedGoogle Scholar
  7. Hodes, R.J., and Singer, A.: MHC restricted recognition by antigen-specific B cells: fact or artefact? Immunology Today 5: 50–54, 1984CrossRefGoogle Scholar
  8. Ivanyi, P., van den Berg-Loonen, E., and de Greeve, P.: Individual mice of one inbred strain produce anti-H-2 and anti-HLA antibodies of different specificities. Tissue Antigens 12: 32–38, 1978PubMedGoogle Scholar
  9. Kast, W.M., de Waal, L.P., and Melief, C.J.M.: Thymus dictates major histocompatibility complex (MHC) specificity and immune response gene phenotype of class II MHC-restricted T cells but not class I MHC-restricted T cells. J. Exp. Med. 160: 1752–1766, 1984PubMedCrossRefGoogle Scholar
  10. Katz, D.H. Skidmore B.J., Katz, L.R., and Bogowitz C.A.: Adaptive differentation of murine lymphocytes. I. Both T and B lymphocytes differentiating in F1’ parental chimeras manifest preferential cooperative activity for partner lymphocytes derived from the same parental type corresponding to the chimeric host. J. Exp. Med. 148: 727–745, 1978PubMedCrossRefGoogle Scholar
  11. Kievits, F., Rocca, A., Opolski, A., Limpens, J., Leupers, T., Kloosterman, T., Boerenkamp, W.P., Pla, M., and Ivanyi, P.: Induction of H-2 specific antibodies by injections of syngeneic Sendai virus-coated cells. Eur. J. Immunol. 17: 27–35, 1987PubMedCrossRefGoogle Scholar
  12. Van Leeuwen, A.E., Goulmy, A., and van Rood, J.J.: Major histocompatibility complex restricted antibody reactivity mainly, but not exclusively, directed against cells from male donors. J. Exp. Med. 150: 1075–1083, 1979PubMedCrossRefGoogle Scholar
  13. Matzinger, P.: A one-receptor view of T-cell behaviour. Nature 292: 497–501, 1981PubMedCrossRefGoogle Scholar
  14. Van Mourik, P., Rivero, R.A., van der Kwast, T.H., Lansdorp, P.M., and Zeijlemaker, W.P.: Density separation of spleen cells increases fusion sequency and yiels of Ig-producing hybridomas. J. Immunol. Methods 68: 45–55, 1984PubMedCrossRefGoogle Scholar
  15. Nisbet-Brown, E., Singh, B., and Diener, E.: Antigen recognition. V. Requirement for histocompatibility between antigen-presenting cell and B-cell in the response to a thymus-dependent antigen, and lack of allogeneic restriction between T and B cells. J. Exp. Med. 154: 676–687, 1981PubMedCrossRefGoogle Scholar
  16. Opolski, A., Kievits, F., and Ivanyi, P.: Polymorphic and autoreactive H-2 specific monoclonal antibodies isolated after injections of syngeneic Sendai virus-coated lymphocytes. Immunogenetics 24: 402–408, 1986PubMedCrossRefGoogle Scholar
  17. Sherman, L.A.: Recognition of conformational determinants on H-2 by cytotoxic T lymphocytes. Nature 297: 511–513, 1982PubMedCrossRefGoogle Scholar
  18. Singer, A., Hathcock, K.S., and Hodes, R.J.: Cellular and genetic control of antibody responses. V. Helper T-cell recognition of H-2 determinants on accessory cells but not B cells. J. Exp. Med. 149: 1208–1229, 1979PubMedCrossRefGoogle Scholar
  19. Sprent, J., and Bruce, J.: Lymphoid function in F1’ parent chimeras. Lack of evidence for adaptive differentation of B cells or antigen-presenting cells. J. Exp. Med. 150: 715–720, 1979PubMedCrossRefGoogle Scholar
  20. Wylie, D.E., Sherman, L.A., and Klinman, N.R.: Participation of the major histocompatibility complex in antibody recognition of viral antigens expressed on infected cells. J. Exp. Med. 155: 403–414, 1982PubMedCrossRefGoogle Scholar
  21. Zinkernagel, R.M., and Doherty, P.C.: MHC-restricted cytotoxic T cells: Studies on the biological role of polymorphic major transplantation antigens determing T cell restriction-specificity. Adv. Immunol. 27: 51–77, 1979PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • F. Kievits
    • 1
  • A. Opolski
    • 2
  • W. J. Boerenkamp
    • 1
  • M. Pla
    • 2
  • P. Ivanyi
    • 1
  1. 1.Central Laboratory of the Netherlands Red Cross Blood Transfusion ServiceLaboratory of Experimental and Clinical Immunology of the University of AmsterdamAmsterdamThe Netherlands
  2. 2.U93, INSERM, Hopital St. LouisParisFrance

Personalised recommendations