Recombination Sites within the I Region of the Mouse H-2 Complex

  • William P. Lafuse
  • Chella S. David
Part of the NATO ASI Series book series (NSSA, volume 144)


Recombinant mouse strains have been important in the development of the genetic map of the H-2 complex and have been used to subdivide the H-2 complex into the K, D, Qa, Tla regions containing genes for class I MHC molecules; I region containing genes for class II MHC molecules; and S region containing genes for class III MHC molecules. A molecular map of the H-2 complex has also been developed with about 1,600 Kb of DNA having been cosmid cloned. The I region has been shown to consist of a 270 Kb segment of DNA that contains six class I genes: Aβ2, Aβ, Aα, Eβ, Eβ2 and Eα (Steinmetz et al. 1982). Over the last few years recombinant mouse strains with crossovers in the I region have been analyzed by DNA restriction fragment analysis and DNA sequencing (Steinmetz et al. 1982, 1986, Kobori et al. 1984, 1986, Lafuse et al. 1986, Lafuse and David, 1986, Begovich et al. 1985, Saha and Cullen 1986, Zimmerer et al. 1987). These studies have shown that recombination within the I region is not random but is limited to two specific locations. These sites which have been termed recombination hot spots are located within the Eβ and Eα genes. In this report we will review these studies and discuss the implication that there are haplotype specific sequences that promote recombination at these two sites. We will also report studies on additional recombinants mapping within the I region.


Globin Gene Recombination Site Haplotype Combination Serological Typing Restriction Fragment Analysis 
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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • William P. Lafuse
    • 1
  • Chella S. David
    • 2
  1. 1.Department of Medical Microbiology and ImmunologyThe Ohio State UniversityColumbusUSA
  2. 2.Department of ImmunologyMayo Clinic and Medical SchoolRochesterUSA

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