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H-2 Antigens pp 611-622 | Cite as

Class I Antigen Expression in Line 1 Cells: Molecular Biology and Immunological Effects

  • John G. Frelinger
  • Karen M. Cerosaletti
  • David W. Bahler
  • Lee W. Harwell
  • Edith M. Lord
Part of the NATO ASI Series book series (NSSA, volume 144)

Abstract

Cultured line 1 murine lung carcinoma cells normally express very low levels of class I H-2 antigens. We have continued our investigations of line 1 lung carcinoma cells to understand the molecular basis of decreased expression of class I H-2 antigens and class I antigen induction with dimethyl sulfoxide (DMSO). We show that line 1, a murine lung carcinoma cell line, has low levels of class I antigens (H-2K, D, and L) because it is deficient in both class I and beta2-microglobulin (B2M) RNAs, and tha. the mRNAs can be coordinately induced with dimethyl sulfoxide (DMSO). Our experiments suggest that interferon can induce surface expression of class I antigens but may act through a different mechanism than DMSO in inducing class I antigens. To further evaluate the regulation of class I expression, H-2Dp genes were transfected into line 1 cells. The transfected H-2 genes appear to be constitutively expressed at much higher levels than are the endogenous class I genes and are associated with endogenous B2M protein. Surface expression of the DP antigens on the transfectants is induced similar to the endogenous H-2d haplotype class I antigens following treatment with DMSO. All the DP transfectants had increased levels of B2M mRNA as well as higher levels of class I RNA when compared to control or untransfected line 1 cells. These results suggest that the regulation of class I and B2M genes is linked and that expression of class I genes can affect expression of B2M genes.

In order to investigate how the expression of class I antigens affects the in vivo growth of this spontaneous tumor, we transplanted the H-2Dp expressing transfectants into mice. Marked differences in tumorigenicity (defined as tumor growth in immunologically competent hosts) were observed between the DP transfected cells and untransfected or control transfected line 1 cells in syngeneic mice only if the animals had previously received injections of irradiated DPtransfectants. Our in vivo results show that increased expression of class I antigens can reduce the growth of tumors like line 1 which lack all class I antigens.

Keywords

Major Histocompatibility Complex Antigen Expression Histocompatibility Antigen Small Lung Cell Carcinoma Cell Haplotype Class 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • John G. Frelinger
    • 1
  • Karen M. Cerosaletti
    • 1
  • David W. Bahler
    • 1
  • Lee W. Harwell
    • 1
  • Edith M. Lord
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Rochester Cancer CenterRochesterUSA

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