Ia Gene Expression in Non-Bone Marrow Derived Cells During Graft Vs Host Disease in the Mouse
We have examined the capacity of non-bone marrow-derived (NBMD) cells to express endogenous Ia genes by evaluating E alpha mRNA expression in various tissues derived from long term bone marrow chimeras. These chimeras were produced in the combination BIO.A(4R) into B10.A and allowed to rest for greater than 100 days before use. Since B10.A(4R) is incapable of transcribing the E gene, E mRNA expression would most likely represent Ia expression by the host B10.A NBMD tissues in these long term chimeras. Our results demonstrate that host E alpha mRNA expression was readily detectable in kidney, lung and thymus, but not in the spleen of chimeric animals. During graft-vs-host disease (GVHD) E alpha expression was enhanced up to 4-fold in kidney and was also detectable in heart extracts as well. These experiments demonstrate that resident host cells, most likely NBMD, in a variety of organs are capable of la gene expression. This expression appears to be both constituitive and inducible. Furthermore, different tissues show a marked quantitative difference in the magnitude of NBMD la expression with kidney showing the highest level.
KeywordsNorthern Blot Analysis Graft Versus Host Disease Alpha Expression Tail Skin Alpha mRNA
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