Abstract
The ultimate function of B lymphocytes is the production and secretion of specific immunoglobulin (Ig). This process is regulated by interactions between cells, cell products, and antigen. Particularly significant are antigen-specific interactions between B and T helper (Th) lymphocytes. Activation of the Th cell in such interactions requires that an antigen-presenting cell, such as a macrophage, dendritic cell, or B cell, present antigen to the Th cell in association with molecules encoded by genes of the class II region of the major histocompatibility complex (MHC). The function of class II antigens in T cell activation has been the subject of intense investigation in recent years (Schwartz, 1985; Germain and Malissen, 1986; Guillet et. al., 1987). There is little understanding, however, of the role of the B cell class II molecule in transmitting a differentiative signal to the B cell itself. This question has been technically difficult to approach, since all antigen-presenting cells express class II antigens. Thus, the unavoidable contamination of uncloned B cells with at least a small percentage of macrophages makes the use of such cells to define the signaling function of B cell Ia problematic. Although long-term culture of untransformed B cells has met with some success (Braun et. al., 1986; Pike and Nossal, 1986), such cell lines are not yet widely available. An alternative approach employs transformed, immortal B cell lines to study the process of B cell differentiation (Arnold et. al., 1986). A major limitation in the study of many B cell tumors is that the specificity of the Ig which they produce is unknown. Therefore, it is difficult to use them to study antigen-specific interactions. However, several clones of transformed murine B cells have been characterized which bear Ig of defined antigenic specificity and are responsive to immunoregulatory signals (Bishop et. al., 1986a). The CH series of murine B cell lymphomas has proven useful in studying B cell differentiation (Haughton et. al., 1986; Bishop and Haughton, 1987).
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References
Arnold, L.W., LoCascio, N.J., Lutz. P.M., Pennell, C.A., Klapper, D., and Haughton, G. Antigen-induced lymphomagenesis: Identification of a murine B cell lymphoma with known antigen specificity. J. Immunol. 131: 2064–2068, 1983.
Arnold, L.W., Lutz, P.M., Pennell, C.A., Bishop, G.A., Corley, R.B., Haughton, G., and LoCascio, N.J. B cell tumors as models of B lymphocyte differentiation. in Cambier, J., Ed. B Lymphocyte Differentiation, CRC Press, Boca Raton, pp. 109–135, 1986.
Benoist, C.O., Mathis, D.J., Kanter, M.R., Williams, V.E.II, and McDevitt, H.O. The murine IA alpha chains E-alpha and A-alpha show a surprising degree of sequence homology. Proc. Natl. Acad. Sci. (USA) 80: 534–538, 1983.
Bishop, G.A., and Haughton, G. Induced differentiation of a transformed clone of Ly-l+ B cells by clonal T cells and antigen. Proc. Natl. Acad. Sci. (USA) 83: 7410–7414, 1986.
Bishop, G.A., Arnold, L.W., and Haughton, G. Antigen-specific B cell tumors of mice. CRC Crit. Rev. Immunol. 6: 105–122, 1986.
Bishop, G.A., and Haughton, G. Use of the CH lymphomas as models of murine B cell differentiation. Immunol. Res. 5: 263–270, 1987.
Braun, J., Citri, Y., Baltimore, D., Forouzanpour, F., King, L., Theranizadeh, K., Bray, M., and Kliever, S. B-Lyl cells: Immortal Ly-l+ B lymphocyte cell lines spontaneously arising in murine splenic cultures. Immunol. Rev. 93: 5–22, 1986.
Brown, M.A., Glimcher, L.A., Nielsen, E.A., Paul, W.E., and Germain, R.N. T-cell recognition of Ia molecules selectively altered by a single amino acid substitution. Science 231: 255–258, 1986.
Chen, Z.Z., McGuire, J.C., Leach, K.L., and Cambier, J.C. Transmembrane signaling through B cell MHC class II molecules: Anti-Ia antibodies induce protein kinase C translocation to the nuclear fraction. J. Immunol. 138: 2345–2352, 1987.
Germain, R.N. and Malissen, B. Analysis of the expression and function of class II MHC-encoded molecules by DNA-mediated gene transfer. Ann. Rev. Immunol. 4: 281–316, 1986.
Guillet, J.G., Lai, M.-Z., Briner, T.J., Buus, S., Sette, A., Grey, H.M., Smith J.A., and Gefter, M.L. Immunological self, nonself discrimination. Science 235: 865–870, 1987.
Haughton, G., Arnold, L.W., Bishop, G.A., and Mercolino, T.J. The CH series of murine B cell lymphomas: Neoplastic analogues of Ly-l+ normal B cells. Immunol. Rev. 93: 35–52, 1986.
Hutchison, C.A.III, Nordeen, S.K., Vogt, K., and Edgell, M.H. A complete library of point substitution mutations in the glucocorticoid element of mouse mammary tumor virus. Proc. Natl. Acad. Sci. (USA) 83: 710–714, 1986.
Lechler, R.I., Ronchese, F., Braunstein, N.S., and Germain, R.N. I-A restricted T cell antigen recognition. Analysis of the roles of Aα and Aβ using DNA-mediated gene transfer. J. Exp. Med. 163: 678–696, 1986.
Macchi, M., Woodward, J., McLaughlin-Taylor, E., Griffin, J., Hood, L., and Frelinger, J.A. Cloning and identification of the H-2DP gene. Immunogenetics 19: 195–204, 1984.
Malissen, B., Price, M.P., Goverman, J.M., McMillan, M., White, J., Kappler, J., Marrack, P., Pierres, A., Pierres, M., and Hood, L. Gene transfer of H-2 class II genes: Antigen presentation by mouse fibroblast and hamster B-cell lines. Cell 36: 319–327, 1984.
Mercolino, T.J., Arnold, L.W., and Haughton, G. Phosphatidyl choline is recognized by a series of Ly-1+ murine B cell lymphomas specific for erythrocyte membranes. J. Exp. Med. 163: 155–165, 1986.
Mulligan, R.C., and Berg, P. Selection for animal cells that express the E. coli. gene coding for xanthine-guanine phospohribosyltransferase. Proc. Natl. Acad. Sci. (USA) 78: 2072–2076, 1981.
Palacios, R., Martinez-Maza, O., and Guy, K. Monoclonal antibodies against HLA-DR antigens replace T helper cells in activation of B lymphocytes. Proc. Natl. Acad. Sci. (USA) 80: 3456–3460, 1983.
Pike, B.L., and Nossal, G.J.V. Growth and differentiation of B cell clones in vitro. in Cambier, J., ED. B Lymphocyte Differentiation, CRC Press, Boca Raton, pp. 79–108, 1986.
Schwartz, R.H. T lymphocyte recognition of antigen in association with gene products of the MHC. Ann. Rev. Immunol. 3: 237–261, 1985.
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Bishop, G.A., Frelinger, J.A. (1987). The Class II Molecule as a Signal Transducer to the B Cell: Cellular and Molecular Approaches. In: David, C.S. (eds) H-2 Antigens. NATO ASI Series, vol 144. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0764-9_51
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DOI: https://doi.org/10.1007/978-1-4757-0764-9_51
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