Activation of I-A Gene Products by Aß Gene Transfection Using Bovine Papilloma Virus — Vector Systems
The bovine papilloma virus (BPV) recombinant which carried class II MHC (A ß d ) gene (pBPAß-d) has been constructed at first. The recombinant plasmid was introduced into the mouse B cell hybridoma by co-transfection method with neomycin resistance gene. Uing this method we could impose into B cell hybridomas many copies of A ß d genes extrachromosomally. The several transfectants which had been introduced only beta chain gene of I-Ad have been revealed to expressed the I-Ad proteins on the cell surface by direct immunofluorescent technique. Moreover these I-Ad molecules could induce the IL-2 production of the I-Ad restricted autoreactive T cell hybridoma. So I-Ad molecules which express on the cell surface of the transfectants are fully functional to the T-cell response. RNA dot-blot analysis has revealed that the cells express not only A ß d gene but also Ad gene. Thus, A ß d gene transfection induced the expression of internally latent Ad gene by trans gene effect.
KeywordsMajor Histocompatibility Complex Gene Transfection Neomycin Resistance Gene High Stringent Condition Trans Gene Effect
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