H-2 Antigens pp 275-281 | Cite as

Activation of I-A Gene Products by Aß Gene Transfection Using Bovine Papilloma Virus — Vector Systems

  • Kenji Okuda
  • Jun Fukushima
  • Susumu Kawamoto
  • Ichiro Aoki
  • Toyozoh Takahashi
Part of the NATO ASI Series book series (NSSA, volume 144)


The bovine papilloma virus (BPV) recombinant which carried class II MHC (A ß d ) gene (pBPAß-d) has been constructed at first. The recombinant plasmid was introduced into the mouse B cell hybridoma by co-transfection method with neomycin resistance gene. Uing this method we could impose into B cell hybridomas many copies of A ß d genes extrachromosomally. The several transfectants which had been introduced only beta chain gene of I-Ad have been revealed to expressed the I-Ad proteins on the cell surface by direct immunofluorescent technique. Moreover these I-Ad molecules could induce the IL-2 production of the I-Ad restricted autoreactive T cell hybridoma. So I-Ad molecules which express on the cell surface of the transfectants are fully functional to the T-cell response. RNA dot-blot analysis has revealed that the cells express not only A ß d gene but also Ad gene. Thus, A ß d gene transfection induced the expression of internally latent Ad gene by trans gene effect.


Major Histocompatibility Complex Gene Transfection Neomycin Resistance Gene High Stringent Condition Trans Gene Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Benacerraf, B. and H.O. McDevitt. Histocompatibility-linked immune response genes: A new class of genes that controls the formation of specific immune responses has been identified. Science 175: 273 (1972).PubMedCrossRefGoogle Scholar
  2. 2.
    Klein, J., A. Juretic, C.N. Baxevanis, and Z.A. Nagy. The traditional and a new version of the mouse H-2 complex. Nature (London) 291: 455 (1981).CrossRefGoogle Scholar
  3. 3.
    Hammerling, G., B. Deak, G. Mauve, U. Hammerling, and H.O. McDevitt. B lymphocyte alloantigens controlled by the I region of the major histocompatibility complex in mice. Immunogenetics 1: 68 (1974).CrossRefGoogle Scholar
  4. 4.
    Schwartz, R. H. T lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex. Ann. Rev. Immunol. 3: 262 (1985).CrossRefGoogle Scholar
  5. 5.
    Kappler, J., B. Skidmore, J. White, and P. Marrack. Antigen-inducible, H-2 restricted, interleukin-2 producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J. Exp. Med. 153: 1198 (1981).PubMedCentralPubMedCrossRefGoogle Scholar
  6. 6.
    Rabourdin-Combe, C., and B. Mach. Expression of HLA-DR antigens at the surface of mouse L cells co-transfected with cloned human genes. Nature 303: 670 (1983).PubMedCrossRefGoogle Scholar
  7. 7.
    Norcross, M. A., D. M. Bentley, D. H. Margulies, and R. N. Germain. Membrane Ia expression and antigen-presenting accessory cell funtion of L cells transfectecd with class II major histocompatibility complex genes. J. Exp. Med. 160: 1316 (1984).PubMedCrossRefGoogle Scholar
  8. 8.
    Jones, P. P., D. B. Murphy, and H. O. McDevitt. Two gene control of the expression of a murine Ia antigen. J. Exp. Med. 148: 925 (1978).PubMedCrossRefGoogle Scholar
  9. 9.
    Shackelford, D. A., L. Lampson, and J. L. Strominger. Separation of three class II antigens from a homozygous human B cell line. J. Immunol. 130: 289 (1983).PubMedGoogle Scholar
  10. 10.
    Kau Man, J. F., C. Auffray, A. J. Korman, D. A. Shackelford, and J. Strominger. The class II molecules of the human and murine major histocompatibility complex. Cell 36: 1 (1984).CrossRefGoogle Scholar
  11. 11.
    Malissen, B., M. Steinmetz, M. McMillan, M. Pierres, and L. Hood. Expression of I-Ak molecules in mouse L cells after DNA-mediated gene transfer. Nature 305: 440 (1983).PubMedCrossRefGoogle Scholar
  12. 12.
    Law, M. F., D. R. Lowy, I. Dvoretzky, and P. M. Howly. Mouse cells transformed by bovine papilloma virus contain only extrachromosomal viral DNA sequences. Proc. Natl. Acad. Sci. USA 78: 2727 (1981).PubMedCrossRefPubMedCentralGoogle Scholar
  13. 13.
    Karin. M., G. Cathala, and M. C. Nguyen-Huu. Expression and regulation of a human metallothionein gene carried on an autonomously replicating shuttle vector. Proc. Natl. Acad. Sci. USA 80: 4040 (1983).PubMedCrossRefPubMedCentralGoogle Scholar
  14. 14.
    Hirt, B. Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Bilo. 26: 365 (1967).CrossRefGoogle Scholar
  15. 15.
    Manser, T., and M. L. Gefter. Isolation of hybridomas expressing a specific heavy chain variable region gene segment by using a screening technique that detects mRNA sequences in whole cell lysates. Proc. Natl. Acad. Sci. USA 81: 2470 (1984).PubMedCrossRefPubMedCentralGoogle Scholar
  16. 16.
    Germain, R. N., and H. Quill. Unexpected expression by L cell transfectants of a unique mixed isotype (Aß: E) class II MHC molecule. Nature 320: 72 (1986)PubMedCrossRefGoogle Scholar
  17. 17.
    Ben-Nun, A., L. H. Glimcher, J. Weiss, and J. G. Seidman. Functional expression of a cloned I-Aßk gene in B lymphoma cells. Science 223: 825 (1984).PubMedCrossRefGoogle Scholar
  18. 18.
    Nir, U., M. D. Walker, and W. J. Rutter. Regulation of insulin I gene expression: Evidence for negative regulation in nonpancreatic cells. Proc. Natl. Acad. Sci. USA 83: 3180 (1986).PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Kenji Okuda
    • 1
  • Jun Fukushima
    • 1
  • Susumu Kawamoto
    • 1
  • Ichiro Aoki
    • 2
  • Toyozoh Takahashi
    • 1
  1. 1.Departments of BacteriologyUniversity School of MedicineYokohamaJapan
  2. 2.Pathology, Yokohama CityUniversity School of MedicineYokohamaJapan

Personalised recommendations